Synaptotagmin-9 in mouse retina

IF 1.1 4区医学 Q4 NEUROSCIENCES

Visual Neuroscience Pub Date : 2024-09-18 DOI:10.1017/s0952523824000026

Chris S. Mesnard, Cassandra L. Hays, Lou E. Townsend, Cody L. Barta, Channabasavaiah B. Gurumurthy, Wallace B. Thoreson

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引用次数: 0

Abstract

Synaptotagmin-9 (Syt9) is a Ca2+ sensor mediating fast synaptic release expressed in various parts of the brain. The presence and role of Syt9 in retina is unknown. We found evidence for Syt9 expression throughout the retina and created mice to conditionally eliminate Syt9 in a cre-dependent manner. We crossed Syt9fl/fl mice with Rho-iCre, HRGP-Cre, and CMV-Cre mice to generate mice in which Syt9 was eliminated from rods (rodSyt9CKO), cones (coneSyt9CKO), or whole animals (CMVSyt9). CMVSyt9 mice showed an increase in scotopic electroretinogram (ERG) b-waves evoked by bright flashes with no change in a-waves. Cone-driven photopic ERG b-waves were not significantly different in CMVSyt9 knockout mice and selective elimination of Syt9 from cones had no effect on ERGs. However, selective elimination from rods decreased scotopic and photopic b-waves as well as oscillatory potentials. These changes occurred only with bright flashes where cone responses contribute. Synaptic release was measured in individual rods by recording anion currents activated by glutamate binding to presynaptic glutamate transporters. Loss of Syt9 from rods had no effect on spontaneous or depolarization-evoked release. Our data show that Syt9 acts at multiple sites in the retina and suggest that it may play a role in regulating transmission of cone signals by rods.

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小鼠视网膜中的突触标记蛋白-9

突触标记蛋白-9（Syt9）是一种介导快速突触释放的 Ca2+ 传感器，在大脑的不同部位都有表达。Syt9在视网膜中的存在和作用尚不清楚。我们发现了Syt9在整个视网膜中表达的证据，并创造了小鼠，以cre依赖的方式有条件地消除Syt9。我们将 Syt9fl/fl 小鼠与 Rho-iCre、HRGP-Cre 和 CMV-Cre 小鼠杂交，产生了从视杆细胞（rodSyt9CKO）、视锥细胞（coneSyt9CKO）或整个动物（CMVSyt9）中消除 Syt9 的小鼠。CMVSyt9 小鼠在强光闪烁诱发的视网膜光图（ERG）b 波中显示出增加，而 a 波没有变化。在 CMVSyt9 基因敲除的小鼠中，锥体驱动的光视 ERG b 波没有显著差异，选择性消除锥体中的 Syt9 对 ERG 没有影响。然而，从视杆细胞中选择性地剔除Syt9会降低视角和光视b波以及振荡电位。这些变化只发生在亮闪光时，而在亮闪光中锥体的反应起作用。通过记录谷氨酸与突触前谷氨酸转运体结合激活的阴离子电流，测量了单个视杆细胞的突触释放。失去视杆细胞中的 Syt9 对自发或去极化诱发的释放没有影响。我们的数据表明，Syt9 在视网膜的多个部位发挥作用，并表明它可能在调节视锥信号通过视杆细胞的传输中发挥作用。

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来源期刊

Visual Neuroscience 医学-神经科学

CiteScore

2.20

自引率

5.30%

发文量

审稿时长

>12 weeks

期刊介绍： Visual Neuroscience is an international journal devoted to the publication of experimental and theoretical research on biological mechanisms of vision. A major goal of publication is to bring together in one journal a broad range of studies that reflect the diversity and originality of all aspects of neuroscience research relating to the visual system. Contributions may address molecular, cellular or systems-level processes in either vertebrate or invertebrate species. The journal publishes work based on a wide range of technical approaches, including molecular genetics, anatomy, physiology, psychophysics and imaging, and utilizing comparative, developmental, theoretical or computational approaches to understand the biology of vision and visuo-motor control. The journal also publishes research seeking to understand disorders of the visual system and strategies for restoring vision. Studies based exclusively on clinical, psychophysiological or behavioral data are welcomed, provided that they address questions concerning neural mechanisms of vision or provide insight into visual dysfunction.