A Pyrazole Partially Induces Brassinosteroid-Related Gene Expression, Leading to Salt Stress Sensitivity

IF 3.9 3区 生物学 Q1 PLANT SCIENCES
Minoru Ueda, Satoshi Takahashi, Junko Ishida, Ayumi Yamagami, Takeshi Nakano, Florian Pünner, Mai Akakabe, Yoshihiro Sohtome, Atsushi J. Nagano, Mikiko Sodeoka, Motoaki Seki
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引用次数: 0

Abstract

Pyrazoles have a broad range of biological properties that make them potentially useful for treating tuberculosis, microbial/fungal infections, and inflammation. In this study, the pyrazole 1,3-diaryl-1H-pyrazol-5-yl)(aryl)methanone (DPAM-1) prepared via catalytic aminooxygenation increased the sensitivity of Arabidopsis to salinity stress. An RNA-seq transcriptome analysis revealed DPAM-1 increased the expression of fewer genes than the coronatine treatment that enhanced salinity stress sensitivity, suggestive of the selective mode of action of DPAM-1. The up-regulated genes included marker genes for brassinosteroid (BR) responses. The responsiveness of BR-related genes, such as CONSTITUTIVE PHOTOMORPHOGENIC DWARF, DWARF4, Small auxin-up RNA_Ac1, and for touch 4 (TCH4)/xyloglucan endotransglucosylase/hydrolase 22 (XTH22), was verified by treatments with brassinolide (BL) and brassinazole (BR biosynthesis inhibitor) and analyses involving the brassinosteroid insensitive 1–5 (bri1-5) mutant carrying a weak allele encoding BRASSINOSTEROID INSENSITIVE 1 receptor kinase under our growth conditions. Among the examined genes, the transcription of only TCH4 increased after the DPAM-1 treatment. Examinations of the bri1-5 mutant indicated that DPAM-1 did not significantly affect the sensitivity of bri1-5 plants to salinity stress, suggesting the increased salinity stress sensitivity following the DPAM-1 treatment was partly mediated by the BR signaling pathway. In the present study, the BL treatment differentially altered the salinity stress tolerance of the Columbia and Wassilewskija accessions. The contribution of BR signaling to salinity stress tolerance during the diversification of Arabidopsis accessions and the potential applicability of DPAM-1 for elucidating the interplay between BR and other phytohormones were assessed.

Abstract Image

一种吡唑能部分诱导芸苔素类固醇相关基因的表达,导致对盐胁迫的敏感性
吡唑具有广泛的生物特性,因此可能有助于治疗结核病、微生物/真菌感染和炎症。在这项研究中,通过催化氨基氧化制备的吡唑-1,3-二芳基-1H-吡唑-5-甲酮(DPAM-1)提高了拟南芥对盐度胁迫的敏感性。RNA-seq 转录组分析表明,DPAM-1 增加的基因表达量少于冠突散囊菌素处理增加的盐胁迫敏感性,这表明了 DPAM-1 的选择性作用模式。上调的基因包括铜绿素(BR)反应的标记基因。BR相关基因,如CONSTITUTIVE PHOTOMORPHOGENIC DWARF、DWARF4、Small auxin-up RNA_Ac1和touch 4 (TCH4)/xyloglucan endotransglucosylase/hydrolase 22 (XTH22)的反应性、通过使用黄铜酸内酯(BL)和黄铜烯唑(BR 生物合成抑制剂)处理,以及在我们的生长条件下对携带编码 BRASSINOSTEROID INSENSITIVE 1 受体激酶弱等位基因的黄铜酸不敏感 1-5 (bri1-5)突变体进行分析,验证了这些基因的活性。在检查的基因中,只有 TCH4 的转录在 DPAM-1 处理后有所增加。对bri1-5突变体的研究表明,DPAM-1并没有显著影响bri1-5植株对盐度胁迫的敏感性,这表明DPAM-1处理后盐度胁迫敏感性的提高部分是由BR信号通路介导的。在本研究中,BL处理不同程度地改变了哥伦比亚和Wassilewskija品种的盐胁迫耐受性。本研究评估了拟南芥品种多样化过程中BR信号转导对盐度胁迫耐受性的贡献,以及DPAM-1在阐明BR和其他植物激素之间相互作用方面的潜在适用性。
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来源期刊
CiteScore
8.40
自引率
6.20%
发文量
312
审稿时长
1.8 months
期刊介绍: The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches. The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress. In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports. The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.
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