Detection of natural pulse waves (PWs) in 3D using high frame rate imaging for anisotropy characterization

Abstract

IntroductionNumerous studies have shown that natural mechanical waves have the potential to assess the elastic properties of the myocardium. When the Aortic and Mitral valves close, a shear wave is produced, which provides insights into tissue stiffness. In addition, the Atrial Kick (AK) generates a wave similar to Pulse Waves (PWs) in arteries, providing another way to assess tissue stiffness. However, tissue anisotropy can also impact PW propagation, which is currently underexplored. This study aims to address this gap by investigating the impact of anisotropy on PW propagation in a phantom.MethodsTube phantoms were created using Polyvinyl Alcohol (PVA). Anisotropy was induced between two sets of two freeze-thaw cycles by stretching and twisting the material. The study first tests and validates the procedure of making helical anisotropic vessel phantoms using the shear wave imaging technique (by estimating the shear wave speed at different probe angles). Using plane wave ultrasound tomography synchronized with a peristaltic pump, 3D high frame rate imaging is performed and used to detect the 3D propagation pattern of PW for each manufactured vessel phantom. Finally, the study attempts to extract the anisotropic coefficient of the vessel using pulse wave propagation angle.ResultsThe Shear wave imaging results obtained for the isotropic vessel show very similar values for each probe angle. On the contrary, the results obtained for the axial anisotropy vessel show a region with a higher shear wave speed at about 0°, corresponding to the long axis of the vessel. Finally, the results obtained for the helical anisotropy depicted increasing shear wave velocity value from −20° to 20°. For the axial phantom, the wavefront of the pulse wave is perpendicular to the long axis of the vessel, while oriented for the helical anisotropic vessels phantom. The pulse wave propagation angle increased with the number of twists made during the vessel manufacturing.DiscussionThe results show that anisotropy can be induced in PVA vessel phantoms by stretching and twisting the material in freeze-thaw cycles. The findings also suggest that vessel anisotropy affects pulse wave propagation angles. Estimating the pulse wave propagation angle may be interesting in characterizing tissue anisotropy in organs where such waves are naturally present.