{"title":"Tracing the origin of the alien pest Cydia pomonella in Algeria through a worldwide comparison of the species’ DNA barcodes","authors":"Tayeb Mahi, Abdelkader Harizia, Tara Canelo, Abdelkader Benguerai, Raul Bonal","doi":"10.1111/afe.12653","DOIUrl":null,"url":null,"abstract":"<jats:list> <jats:list-item>DNA barcodes (i.e., gene sequences used as identifiers for species identification) constitute a very useful tool in invasive pest research. Using them, we studied, for the first time, the origin and population structure of the alien <jats:italic>Cydia pomonella</jats:italic> in Africa, a major apple pest.</jats:list-item> <jats:list-item>We sequenced a fragment of the mitochondrial gene Cytochrome c oxidase subunit I (COI) from <jats:italic>C. pomonella</jats:italic> caterpillars collected in northern Algeria and compared them with DNA barcodes from the rest of the world available at public repositories.</jats:list-item> <jats:list-item>The phylogeny built upon that COI database supports the European origin of the species: haplotypes at the base of the phylogeny were registered in this continent. In Algeria, the results suggest that this pest could have been introduced from Europe, with two unique African mutations probably favoured by <jats:italic>C. pomonella</jats:italic> multivoltinism and large population sizes.</jats:list-item> <jats:list-item>Population genetic analyses at three Algerian localities showed neither structure nor founder effects. However, to confirm the underlying process of the observed population patterns, it is necessary to perform analyses using genes with higher mutation rates.</jats:list-item> <jats:list-item>Compared to higher resolution markers, mitochondrial DNA barcodes are still a cost‐effective tool for taxonomic determination of unknown specimens and, sometimes, may also allow a preliminary tracing of its origin. This is crucial to detect and stop the spread of invasive pests (i.e., borders). We encourage further studies including functional genes to assess whether any mutations are promoting <jats:italic>C. pomonella</jats:italic> adaptation to the Northern Africa environment.</jats:list-item> </jats:list>","PeriodicalId":7454,"journal":{"name":"Agricultural and Forest Entomology","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agricultural and Forest Entomology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/afe.12653","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
DNA barcodes (i.e., gene sequences used as identifiers for species identification) constitute a very useful tool in invasive pest research. Using them, we studied, for the first time, the origin and population structure of the alien Cydia pomonella in Africa, a major apple pest.We sequenced a fragment of the mitochondrial gene Cytochrome c oxidase subunit I (COI) from C. pomonella caterpillars collected in northern Algeria and compared them with DNA barcodes from the rest of the world available at public repositories.The phylogeny built upon that COI database supports the European origin of the species: haplotypes at the base of the phylogeny were registered in this continent. In Algeria, the results suggest that this pest could have been introduced from Europe, with two unique African mutations probably favoured by C. pomonella multivoltinism and large population sizes.Population genetic analyses at three Algerian localities showed neither structure nor founder effects. However, to confirm the underlying process of the observed population patterns, it is necessary to perform analyses using genes with higher mutation rates.Compared to higher resolution markers, mitochondrial DNA barcodes are still a cost‐effective tool for taxonomic determination of unknown specimens and, sometimes, may also allow a preliminary tracing of its origin. This is crucial to detect and stop the spread of invasive pests (i.e., borders). We encourage further studies including functional genes to assess whether any mutations are promoting C. pomonella adaptation to the Northern Africa environment.
DNA 条形码(即用于物种鉴定的基因序列)是入侵害虫研究中非常有用的工具。利用条形码,我们首次研究了苹果的主要害虫--非洲外来害虫Cydia pomonella的起源和种群结构。我们对在阿尔及利亚北部采集到的单胞矢车菊毛虫线粒体基因细胞色素 c 氧化酶亚单位 I(COI)片段进行了测序,并将其与公共资料库中来自世界其他地区的 DNA 条形码进行了比较。根据 COI 数据库建立的系统发育支持该物种起源于欧洲:系统发育基础上的单倍型在欧洲大陆有记录。在阿尔及利亚,研究结果表明,这种害虫可能是从欧洲引入的,其两个独特的非洲变异可能是由于 C. pomonella 的多伏性和庞大的种群规模而产生的。对阿尔及利亚三个地方的种群遗传分析表明,既没有结构效应,也没有始祖效应。不过,要确认观察到的种群模式的基本过程,有必要使用突变率较高的基因进行分析。与分辨率更高的标记物相比,线粒体 DNA 条形码仍是对未知标本进行分类鉴定的一种经济有效的工具,有时还能对标本的来源进行初步追踪。这对于检测和阻止入侵害虫(即边界害虫)的传播至关重要。我们鼓励开展包括功能基因在内的进一步研究,以评估是否有突变促进了 C. pomonella 对北非环境的适应。
期刊介绍:
Agricultural and Forest Entomology provides a multi-disciplinary and international forum in which researchers can present their work on all aspects of agricultural and forest entomology to other researchers, policy makers and professionals.
The Journal welcomes primary research papers, reviews and short communications on entomological research relevant to the control of insect and other arthropod pests. We invite high quality original research papers on the biology, population dynamics, impact and management of pests of the full range of forest, agricultural and horticultural crops.