Efficiency of the zinc chelator 1,10-phenanthroline for assisted oocyte activation following ICSI in pigs

IF 1.8 4区生物学 Q3 DEVELOPMENTAL BIOLOGY

Reproduction, fertility, and development Pub Date : 2024-09-13 DOI:10.1071/rd24129

Olinda Briski, Juan P. Cabeza, Daniel F. Salamone, Rafael Fernández-Martin, Andrés Gambini

{"title":"Efficiency of the zinc chelator 1,10-phenanthroline for assisted oocyte activation following ICSI in pigs","authors":"Olinda Briski, Juan P. Cabeza, Daniel F. Salamone, Rafael Fernández-Martin, Andrés Gambini","doi":"10.1071/rd24129","DOIUrl":null,"url":null,"abstract":" ContextIn vitro embryo production in pigs is an important tool for advancing biomedical research. Intracytoplasmic sperm injection (ICSI) circumvents the polyspermy problems associated with conventional IVF in porcine. However, the suboptimal efficiency for ICSI in pigs requires new strategies to increase blastocyst formation rates. AimTo investigate novel methods for assisted activation using the zinc chelator 1,10-phenanthroline (PHEN), and to improve embryo developmental competence and quality of ICSI porcine blastocyst. MethodsICSI embryos were treated with PHEN after or before sperm injection, recording pronuclear formation, blastocyst rate and the expression of SMARCA4, OCT4, SOX2 and CDX2. Key resultsNeither electrical nor PHEN significantly improves pronuclear formation rates before or after ICSI. Following in vitro culture to the blastocyst stage, no significant differences were observed in developmental rates among the groups. Moreover, the use of PHEN did not alter the total cell number or the expression of OCT4, SOX2 and CDX2 in pig ICSI blastocysts. ConclusionsAssisted oocyte activation with PHEN does not affect the preimplantation development of ICSI-derived pig embryos. ImplicationsThese results hold significance in refining and advancing the application of assisted oocyte activation techniques. They offer insights into addressing fertility issues and propelling advancements in human and animal reproductive medicine.","PeriodicalId":20932,"journal":{"name":"Reproduction, fertility, and development","volume":"31 1","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproduction, fertility, and development","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1071/rd24129","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Context

In vitro embryo production in pigs is an important tool for advancing biomedical research. Intracytoplasmic sperm injection (ICSI) circumvents the polyspermy problems associated with conventional IVF in porcine. However, the suboptimal efficiency for ICSI in pigs requires new strategies to increase blastocyst formation rates.

Aim

To investigate novel methods for assisted activation using the zinc chelator 1,10-phenanthroline (PHEN), and to improve embryo developmental competence and quality of ICSI porcine blastocyst.

Methods

ICSI embryos were treated with PHEN after or before sperm injection, recording pronuclear formation, blastocyst rate and the expression of SMARCA4, OCT4, SOX2 and CDX2.

Key results

Neither electrical nor PHEN significantly improves pronuclear formation rates before or after ICSI. Following in vitro culture to the blastocyst stage, no significant differences were observed in developmental rates among the groups. Moreover, the use of PHEN did not alter the total cell number or the expression of OCT4, SOX2 and CDX2 in pig ICSI blastocysts.

Conclusions

Assisted oocyte activation with PHEN does not affect the preimplantation development of ICSI-derived pig embryos.

Implications

These results hold significance in refining and advancing the application of assisted oocyte activation techniques. They offer insights into addressing fertility issues and propelling advancements in human and animal reproductive medicine.

查看原文本刊更多论文

锌螯合剂 1,10-菲罗啉在猪卵胞浆内单精子显微注射后辅助激活卵母细胞的效率

背景猪体外胚胎生产是推动生物医学研究的重要工具。卵胞浆内单精子显微注射（ICSI）避免了传统猪体外受精中的多精子问题。然而，猪的卵胞浆内单精子显微注射（ICSI）效率并不理想，因此需要新的策略来提高囊胚形成率。目的研究使用锌螯合剂 1,10-菲罗啉（PHEN）进行辅助激活的新方法，并提高猪 ICSI 囊胚的胚胎发育能力和质量。方法在精子注射后或注射前用 PHEN 处理 ICSI 胚胎，记录前核形成、囊胚率以及 SMARCA4、OCT4、SOX2 和 CDX2 的表达。主要结果在卵胞浆内单精子显微注射（ICSI）之前或之后，电疗和 PHEN 都不能明显提高无核形成率。在体外培养至囊胚期后，各组的发育率无明显差异。此外，使用 PHEN 不会改变猪 ICSI 囊胚的细胞总数或 OCT4、SOX2 和 CDX2 的表达。结论使用 PHEN 辅助卵母细胞活化不会影响 ICSI 衍生猪胚胎的植入前发育。意义这些结果对完善和推进辅助卵母细胞活化技术的应用具有重要意义。它们为解决生育问题和推动人类与动物生殖医学的发展提供了启示。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Reproduction, fertility, and development 生物-动物学

CiteScore

2.10

自引率

10.50%

发文量

317

审稿时长

2 months

期刊介绍： Reproduction, Fertility and Development is an international journal for the publication of original and significant contributions on vertebrate reproductive and developmental biology. Subject areas include, but are not limited to: physiology, biochemistry, cell and molecular biology, endocrinology, genetics and epigenetics, behaviour, immunology and the development of reproductive technologies in humans, livestock and wildlife, and in pest management. Reproduction, Fertility and Development is a valuable resource for research scientists working in industry or academia on reproductive and developmental biology, clinicians and veterinarians interested in the basic science underlying their disciplines, and students. Reproduction, Fertility and Development is the official journal of the International Embryo Technology Society and the Society for Reproductive Biology. Reproduction, Fertility and Development is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.