The design and fabrication of thermoplastic microfluidic chips with integrated micropillars for particle separation

Abstract

Microfluidic technology utilizing the deterministic lateral displacement (DLD) method holds significant promise for efficiently separating micro-particles and biological cells. Despite the notable high throughput advantages associated with DLD chips, their widespread application is impeded by the substantial manufacturing costs of tens of thousands of micropillars. This study aims to explore the feasibility of employing the injection molding method for the mass production of DLD microfluidic chips. A multistage DLD chip with varied critical diameters was designed to isolate white blood cells from the human whole blood. The separation effectiveness was verified with the polydimethylsiloxane chip fabricated by standard soft lithography. Subsequently, nickel mold inserts were electroformed to fabricate thermoplastic DLD chips via the injection molding. The replication quality of micropillars under different molding parameters was studied. The capability of injection-molded chips to effectively achieve particle separation was validated. Results showed that thermoplastic chips with good replication quality were obtained, providing a scale-up production strategy for fabricating polymer-based microfluidic chips for disposable separation applications.