CircBIRC6 affects prostate cancer progression by regulating miR-574-5p and DNAJB1.

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-09-11 DOI:10.1080/15384047.2024.2399363

Bin Zhao,Jinye Yang,Fengming Ran,Yuanlong Shi,Libo Yang,Yuanpeng Duan,Zhiyu Shi,Xin Li,Jianpeng Zhang,Zhiyao Li,Jiansong Wang

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引用次数: 0

Abstract

BACKGROUND Prostate cancer (PCa) is among the three main types of cancer. Although prostate-specific antigen (PSA) is routinely tested, it has disadvantages, such as poor prognostic ability. Therefore, finding more PCa markers and therapeutic targets remains a subject of study. CircRNAs have been found to have regulatory roles in various diseases, such as diabetes, Central Nervous System (CNS) neuropathy, etc. where their application in cancer is even more valuable. Therefore, this paper aims to search for differentially expressed circRNAs in PCa and find downstream targeting pathways related to autophagy. METHOD By detecting the expression of circRNA in the samples, hsa_circ_0119816 was finally identified as the research target. The properties of circRNA were verified by RNase R, actinomycin D, and fluorescence in situ hybridization (FISH). The downstream target miRNAs and target proteins were predicted by an online database, and the targeting relationship was verified using dual luciferase and RNA Immunoprecipitation. The effects of circRNAs and their downstream signalling pathways on prostate cancer cell proliferation, migration, EMT and autophagy were examined by CCK-8, Transwell, immunofluorescence and Western blotting. RESULTS CircBIRC6 is highly expressed in prostate cancer samples. Knockdown of its expression inhibits cell proliferation, invasion, EMT and autophagy and promotes apoptosis. CircBIRC6/miRNA-574-5p/DNAJB1 is a molecular axis that regulates prostate cancer cells.

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CircBIRC6 通过调控 miR-574-5p 和 DNAJB1 影响前列腺癌的进展。

背景前列腺癌（PCa）是三大癌症之一。虽然前列腺特异性抗原（PSA）是常规检测指标，但它也有缺点，如预后能力差。因此，寻找更多的 PCa 标志物和治疗靶点仍是一个研究课题。目前已发现 CircRNAs 在糖尿病、中枢神经系统（CNS）神经病变等多种疾病中具有调控作用，其在癌症中的应用价值更高。因此，本文旨在寻找 PCa 中差异表达的 circRNA，并找到与自噬相关的下游靶向通路。方法通过检测样本中 circRNA 的表达，最终确定 hsa_circ_0119816 为研究靶点。通过 RNase R、放线菌素 D 和荧光原位杂交（FISH）验证了 circRNA 的特性。通过在线数据库预测了下游靶miRNA和靶蛋白，并利用双荧光素酶和RNA免疫沉淀验证了靶向关系。通过CCK-8、Transwell、免疫荧光和Western印迹检测了circRNAs及其下游信号通路对前列腺癌细胞增殖、迁移、EMT和自噬的影响。结果CircBIRC6在前列腺癌样本中高表达，抑制其表达可抑制细胞增殖、侵袭、EMT和自噬，并促进细胞凋亡。CircBIRC6/miRNA-574-5p/DNAJB1是调控前列腺癌细胞的分子轴。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.