Effect of urea on the linear and nonlinear rheological properties of human serum albumin hydrogels

IF 2.3 3区 工程技术 Q2 MECHANICS
Tochukwu Olunna Nnyigide, Osita Sunday Nnyigide, Kyu Hyun
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引用次数: 0

Abstract

We report the linear and nonlinear rheological properties of human serum albumin (HSA) hydrogels with and without urea using small (SAOS) and large (LAOS) amplitude oscillatory shear tests. In SAOS tests, pure HSA and HSA-urea hydrogels exhibited a predominantly solid-like behavior (G' ≫ G''), with viscoelastic properties proportional to HSA concentrations. As urea concentration increased, the viscoelastic properties of the hydrogels decreased and the frequency dependence declined, indicating a sparser cross-linking network. Under LAOS flow, both pure HSA and HSA-urea hydrogels exhibited intra- and inter-cycle strain-stiffening, which became more pronounced with increasing urea concentration. The presence of urea delayed the onset of the nonlinear behavior in HSA-urea hydrogels, in proportion to the urea concentration, decreasing the hydrogel strength. The degree of nonlinearity, quantified by the intrinsic nonlinear parameter Q0 from Fourier-transform rheology, decreased with increasing urea concentration. Additionally, critical strain amplitudes obtained from LAOS tests indicated that the yield strain of the HSA-urea hydrogels increased with urea concentration. The intracycle behavior was analyzed by sequence of physical processes methods. Molecular dynamics simulations were performed to analyze the hydrogels behavior at the atomic level. Significant changes in the hydrogel network were attributed to the efficient insertion of urea into the HSA hydrogen bond network, which forms the cross-linking network. Thus, the hydrogen bonding between urea and HSA, as well as urea’s contribution to HSA denaturation, affects the gelation, linear, and nonlinear viscoelastic properties of the HSA hydrogels.

Abstract Image

尿素对人血清白蛋白水凝胶线性和非线性流变特性的影响
我们利用小振幅(SAOS)和大振幅(LAOS)振荡剪切试验,报告了含尿素和不含尿素的人血清白蛋白(HSA)水凝胶的线性和非线性流变特性。在 SAOS 试验中,纯 HSA 和 HSA- 尿素水凝胶主要表现出类似固体的行为(G' ≫ G''),其粘弹性与 HSA 浓度成正比。随着尿素浓度的增加,水凝胶的粘弹性降低,频率依赖性下降,表明交联网络更加稀疏。在 LAOS 流动条件下,纯 HSA 和 HSA- 尿素水凝胶都表现出周期内和周期间的应变刚性,这种刚性随着尿素浓度的增加而变得更加明显。尿素的存在延迟了 HSA- 尿素水凝胶非线性行为的发生,与尿素浓度成正比,从而降低了水凝胶的强度。非线性程度(用傅立叶变换流变学的本征非线性参数 Q0 量化)随着尿素浓度的增加而降低。此外,LAOS 测试获得的临界应变振幅表明,HSA-尿素水凝胶的屈服应力随尿素浓度的增加而增加。通过物理过程序列方法分析了循环内行为。分子动力学模拟从原子水平分析了水凝胶的行为。水凝胶网络的显著变化归因于脲有效地插入了 HSA 氢键网络,从而形成了交联网络。因此,脲和 HSA 之间的氢键以及脲对 HSA 变性的贡献影响了 HSA 水凝胶的凝胶化、线性和非线性粘弹性能。
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来源期刊
Rheologica Acta
Rheologica Acta 物理-力学
CiteScore
4.60
自引率
8.70%
发文量
55
审稿时长
3 months
期刊介绍: "Rheologica Acta is the official journal of The European Society of Rheology. The aim of the journal is to advance the science of rheology, by publishing high quality peer reviewed articles, invited reviews and peer reviewed short communications. The Scope of Rheologica Acta includes: - Advances in rheometrical and rheo-physical techniques, rheo-optics, microrheology - Rheology of soft matter systems, including polymer melts and solutions, colloidal dispersions, cement, ceramics, glasses, gels, emulsions, surfactant systems, liquid crystals, biomaterials and food. - Rheology of Solids, chemo-rheology - Electro and magnetorheology - Theory of rheology - Non-Newtonian fluid mechanics, complex fluids in microfluidic devices and flow instabilities - Interfacial rheology Rheologica Acta aims to publish papers which represent a substantial advance in the field, mere data reports or incremental work will not be considered. Priority will be given to papers that are methodological in nature and are beneficial to a wide range of material classes. It should also be noted that the list of topics given above is meant to be representative, not exhaustive. The editors welcome feedback on the journal and suggestions for reviews and comments."
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