Scrutinizing the effect of additive and synergistic antibiotics against carbapenem-resistant Pseudomonas aeruginosa

IF 2.1 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Open Chemistry Pub Date : 2024-09-12 DOI:10.1515/chem-2024-0074

Fatima Siddiqui, Nureen Zahra, Basit Zeshan, Ayaz Ali Khan, Abid Sarwar, Tariq Aziz, Metab Alharbi, Abdulrahman Alshammari

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引用次数: 0

Abstract

Pseudomonas aeruginosa causes many infections worldwide. Multidrug-resistant bacteria are mostly watched in hospital-acquired infections and are linked with high mortality rates. This study aims to find a possible cure for P. aeruginosa by using an additive and synergistic combination of drugs. The Carbapenem-resistant P. aeruginosa were isolated and identified by using clinical and laboratory standards institute protocols and their antibiotic susceptibility testing was performed by using disc and well diffusion method. In computational analysis, all of the ligands and proteins were selected and docking was done using PYRX. Hundred samples were taken of isolated Gram-negative bacteria of which 25% were P. aeruginosa. Additive combinations (meropenem [MPM] + tobramycin [TOB] was 100% sensitive and imipenem + TOB was also 100% sensitive) and synergistic combinations (MPM + ciprofloxacin were 90% sensitive and amikacin and imipenem) were 100% sensitive according to CLSI protocol. In computational analysis, TOB gave the highest binding energy of −11.4, whereas MPM shows a binding energy of −9.3. In conclusion, Additive and synergistic combination of drugs showed good antibacterial activity against Pseudomonas aeruginosa. Hence it can be used as a good treatment option for P. aeruginosa.

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研究添加抗生素和协同抗生素对耐碳青霉烯类铜绿假单胞菌的影响

铜绿假单胞菌在全球引起多种感染。耐多药细菌多见于医院获得性感染，并与高死亡率有关。本研究旨在通过使用添加剂和协同增效组合药物，找到治疗铜绿假单胞菌的可能方法。采用临床和实验室标准研究所的规程对耐碳青霉烯类的铜绿假单胞菌进行了分离和鉴定，并采用盘式和井式扩散法对其进行了抗生素药敏试验。在计算分析中，选择了所有配体和蛋白质，并使用PYRX进行了对接。从分离的革兰氏阴性菌中提取了 100 个样本，其中 25% 为铜绿假单胞菌。根据 CLSI 协议，添加组合（美罗培南[MPM] +妥布霉素[TOB] 的敏感性为 100%，亚胺培南 + TOB 的敏感性也是 100%）和协同组合（MPM + 环丙沙星的敏感性为 90%，阿米卡星和亚胺培南的敏感性为 100%）的敏感性均为 100%。在计算分析中，TOB 的结合能最高，为 -11.4，而 MPM 的结合能为 -9.3。总之，添加型和协同型药物组合对铜绿假单胞菌具有良好的抗菌活性。因此，它可以作为治疗铜绿假单胞菌的一种良好选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Open Chemistry CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

3.80

自引率

4.30%

发文量

审稿时长

6 weeks

期刊介绍： Open Chemistry is a peer-reviewed, open access journal that publishes original research, reviews and short communications in the fields of chemistry in an ongoing way. The central goal is to provide a hub for researchers working across all subjects to present their discoveries, and to be a forum for the discussion of the important issues in the field. The journal is the premier source for cutting edge research in fundamental chemistry and it provides high quality peer review services for its authors across the world. Moreover, it allows for libraries everywhere to avoid subscribing to multiple local publications, and to receive instead all the necessary chemistry research from a single source available to the entire scientific community.