Fishing for Estuarine Oomycetes

Diversity Pub Date : 2024-09-01 DOI:10.3390/d16090530

Petya Koeva Christova

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Abstract

Oomycetes are water molds that are frequently isolated during a survey of waterways. Biodiversity of oomycetes in the estuary region of the Veleka River in Bulgaria was investigated in 2021. A total number of 32 isolates were derived using the baiting method. Species identification based on sequencing of the ITS region divided them into four different genera: Phytophthora (P.), Phytopythium (Pp.), Pythium (Py.) and Elongisporangium (E.). The most abundant species in the studied region was P. lacustris (sixteen isolates), followed by P. honggalleglyana (nine isolates). P. bilorbang and P. inundata were represented by only one isolate each and were recognized for the first time in Bulgaria. The genus Phytopythium was presented by two isolates that belong to different species, Pp. litorale and Pp. citrinum. In the obtained collection, the genera Pythium and Elongisporangium were represented by only one species each, Py. angustatum (one isolate) and E. anandrum (two isolates), respectively. Colony morphology of the eight collected oomycete species was characterized by cultivation of selected isolates on three different media. Potential host species of the isolated estuarine oomycetes were estimated by pathogenicity tests conducted with sixteen plants from ten diverse families. P. lacustris and P. honggalleglyana demonstrated a higher aggressiveness among Phytophthora isolates, whereas P. bilorbang and P. inundata showed less ability to infect the tested plant species. Similar pathogenicity and a potential host range for both Phytopythium species were observed. Less aggressive against analyzed plants in this study were Py. angustatum and E. anandrum.

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捕捞河口卵菌

卵菌是一种水霉菌，在水道调查中经常被分离出来。2021 年，研究人员对保加利亚 Veleka 河河口地区的卵菌生物多样性进行了调查。采用诱饵法共获得 32 个分离物。根据 ITS 区域测序进行的物种鉴定将其分为四个不同的属：Phytophthora (P.)、Phytopythium (Pp.)、Pythium (Py.) 和 Elongisporangium (E.)。在研究区域中最多的物种是 P. lacustris（16 个分离株），其次是 P. honggalleglyana（9 个分离株）。P. bilorbang 和 P. inundata 各只有一个分离株，是首次在保加利亚发现。Phytopythium 属有两个属于不同种的分离物，分别是 Pp.在收集到的样本中，Pythium 属和 Elongisporangium 属各只有一个种，分别是 Py. angustatum（一个分离株）和 E. anandrum（两个分离株）。通过在三种不同培养基上培养选定的分离物，对收集到的八个卵菌种类的菌落形态进行了鉴定。通过对 10 个不同科的 16 种植物进行致病性试验，估计了分离出的河口卵菌的潜在宿主物种。在 Phytophthora 分离物中，P. lacustris 和 P. honggalleglyana 表现出较高的侵染性，而 P. bilorbang 和 P. inundata 感染受测植物物种的能力较弱。两种疫霉菌的致病性和潜在寄主范围相似。在本研究中，Py. angustatum 和 E. anandrum 对分析植物的侵染能力较弱。

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