Clinical significance of T helper cell subsets in the peripheral blood and bone marrow of patients with multiple myeloma

IF 8.3 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

ACS Applied Materials & Interfaces Pub Date : 2024-09-11 DOI:10.3389/fimmu.2024.1445530

Liangjun Zhang, Huixiu Zhong, Jiwen Fan, Jiansen Mao, Yi Li

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Abstract

BackgroundT helper (Th) cell subsets primarily assist B cells in differentiating into plasma cells in the germinal center. The mechanism of malignant transformation of plasma cells is an important target for the clinical treatment of MM; however, the mechanism remains unclear.MethodsWe collected the peripheral blood (PB) and bone marrow (BM) samples of 33 patients with MM. In addition, the PB was also collected from 25 normal healthy controls (HCs). We analyzed the percentages of Th cell subsets in the PB and BM samples of patients with MM.ResultsTfh/CD4+ were positively correlated with the proportion of myeloma cells in the BM and PB samples (r = 0.592, P = 0.002 and r = 0.510, P = 0.010 respectively), and showed a strong correlation between the BM and PB samples (r = 0.6559, P = 0.0095). In the PB samples, the percentages of Th2/CD4+ and Tfh2/Tfh cells were significantly lower in patients with MM than in HCs (P = 0.00013 and P = 0.0004, respectively), whereas the percentage of Th17/CD4+ and Tfh17/Tfh was significantly higher in newly diagnosed patients with MM than in HCs (P = 0.0037 and P = 0.03, respectively), and all these cells showed a good predictive value for MM (area under the curve [AUC] 0.781, = 0.792, = 0.837, and 0.723 respectively). In the PB samples, all subsets of PD-1+ICOS- Tfh showed a noticeable downward trend in MM from newly diagnosed to non-remission and remission groups. In contrast, all subsets of PD-1-ICOS+ Tfh increased gradually.ConclusionTh cell subsets play an important role in the occurrence and development of MM and may provide a fundamental basis for identifying new immunotherapy targets and prognosis.

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多发性骨髓瘤患者外周血和骨髓中 T 辅助细胞亚群的临床意义

背景T辅助细胞亚群主要协助B细胞在生殖中心分化为浆细胞。浆细胞恶性转化的机制是 MM 临床治疗的一个重要靶点；然而，其机制仍不清楚。方法我们收集了 33 名 MM 患者的外周血（PB）和骨髓（BM）样本。此外，我们还采集了 25 名正常健康对照者（HCs）的外周血样本。结果Tfh/CD4+与骨髓和外周血样本中骨髓瘤细胞的比例呈正相关（分别为r = 0.592，P = 0.002和r = 0.510，P = 0.010），并且在骨髓和外周血样本之间显示出很强的相关性（r = 0.6559，P = 0.0095）。在肺结核样本中，MM 患者的 Th2/CD4+ 和 Tfh2/Tfh 细胞百分比显著低于 HCs（分别为 P = 0.00013 和 P = 0.0004），而新诊断的 MM 患者的 Th17/CD4+ 和 Tfh17/Tfh 百分比显著高于 HCs（分别为 P = 0.0037 和 P = 0.03），而且所有这些细胞对 MM 都有很好的预测价值（曲线下面积 [AUC] 分别为 0.781、= 0.792、= 0.837 和 0.723）。在 PB 样本中，PD-1+ICOS- Tfh 的所有亚群在 MM 新诊断组、非缓解组和缓解组中都显示出明显的下降趋势。结论Th 细胞亚群在 MM 的发生和发展中起着重要作用，可为确定新的免疫治疗靶点和预后提供基本依据。

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来源期刊

ACS Applied Materials & Interfaces 工程技术-材料科学：综合

CiteScore

16.00

自引率

6.30%

发文量

4978

审稿时长

1.8 months

期刊介绍： ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.