Molecular Landscape of Bolting in Spinach Explored Through Gene Expression Profiling

IF 3.9 3区生物学 Q1 PLANT SCIENCES

Journal of Plant Growth Regulation Pub Date : 2024-08-19 DOI:10.1007/s00344-024-11457-x

Marziye Amini, Zahra Hasanpour, Aboozar Soorni, Rahim Mehrabi

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Abstract

This study aims to investigate the molecular mechanisms governing bolting in spinach (Spinacia oleracea) by analyzing gene expression patterns in key regulatory pathways. Two cultivars, Kashan (early bolting) and Virofly (late-bolting), were analyzed using quantitative real-time polymerase chain reaction (qRT-PCR) at different developmental stages. The photoperiod pathway genes Cryptochrome2 (CRY2) and Flavin-binding kelch repeat F-box protein (FKF1) showed distinct expression dynamics, highlighting their roles in the vegetative-to-reproductive transition. Aging pathway genes Topless (TPL), Squamosa promoter binding protein5 (SPL5), SPL15, and Alpha-ketoglutarate-dependent dioxygenase AlkB-like (AlkB) revealed complex expression patterns, with SPL5 and SPL15 differing significantly in Virofly. Circadian pathway genes LATE ELONGATED HYPOCOTYL (LHY) and TIMING OF CAB EXPRESSION 1 (TOC1) showed distinct patterns, particularly LHY in Kashan. Autonomous pathway genes LUMINIDEPENDENS (LD) and FLOWERING LOCUS D (FLD) also varied, with LD higher in Kashan at the eight-leaf stage, while FLD was generally elevated. These findings provide insights into the interactions among photoperiod, aging, circadian, and autonomous pathways, suggesting regulatory mechanisms influencing bolting time. Further research into these pathways could enhance spinach breeding for improved yield and quality.

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通过基因表达谱分析探索菠菜茎枯的分子景观

本研究旨在通过分析关键调控途径的基因表达模式，研究菠菜（Spinacia oleracea）萌芽的分子机制。研究使用实时定量聚合酶链式反应（qRT-PCR）分析了两个栽培品种--Kashan（早勃）和 Virofly（晚勃）在不同发育阶段的基因表达情况。光周期途径基因隐色素2（CRY2）和黄素结合开尔奇重复F-盒蛋白（FKF1）表现出不同的表达动态，突出了它们在无性繁殖向生殖过渡中的作用。衰老途径基因Topless（TPL）、Squamosa启动子结合蛋白5（SPL5）、SPL15和α-酮戊二酸依赖性二氧酶AlkB-like（AlkB）显示出复杂的表达模式，其中SPL5和SPL15在Virofly中差异显著。昼夜节律途径基因 LATE ELONGATED HYPOCOTYL（LHY）和 TIMING OF CAB EXPRESSION 1（TOC1）显示出不同的表达模式，尤其是 LHY 在卡尚的表达模式。自主通路基因 LUMINIDEPENDENS（LD）和 FLOWERING LOCUS D（FLD）也各不相同，卡尚的 LD 在八叶期较高，而 FLD 则普遍升高。这些发现深入揭示了光周期、衰老、昼夜节律和自主途径之间的相互作用，提出了影响萌芽时间的调控机制。对这些途径的进一步研究可促进菠菜育种，提高产量和质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Plant Growth Regulation 生物-植物科学

CiteScore

8.40

自引率

6.20%

发文量

312

审稿时长

1.8 months

期刊介绍： The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches. The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress. In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports. The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.