A cold‐adapted and robust alkaline lipase from Marinobacter nanhaiticus boosts laundry detergent performance

IF 1.6 4区工程技术 Q3 CHEMISTRY, APPLIED

Journal of Surfactants and Detergents Pub Date : 2024-08-13 DOI:10.1002/jsde.12793

Qinzhou Cai, Huifang Zhang, Bingshan Zhao, Lei Ren, Yonghua Wang, Fanghua Wang

引用次数: 0

Abstract

Cold‐adapted, alkali‐stable lipases with surfactant tolerance are highly sought after in the detergent industry. In this study, we recombinantly expressed and characterized a novel lipase from Marinobacter nanhaiticus (MNL). The purified MNL exhibited high stability within a temperature range of 10–30°C and pH values of 8–10, demonstrating optimal activity at 20°C and pH 8. MNL has a preference for substrates with medium chains with the best activity being 3768.6 U/mg using p‐nitrophenyl decanoate as a substrate. Remarkably, MNL showed enhanced enzymatic activity in the presence of ionic surfactants and displayed notable resilience when exposed to proteases. Washing performance analysis further revealed MNL's high proficiency in removing oil‐based stains from fabrics. Collectively, these results suggest that MNL holds significant promise as a valuable component in laundry detergent formulations.

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来自南海马林杆菌的一种适应低温且强健的碱性脂肪酶可提高洗衣粉性能

在洗涤剂行业中，具有耐冷性、碱稳定性和表面活性剂耐受性的脂肪酶备受青睐。在这项研究中，我们重组表达并鉴定了一种来自南海马林杆菌（Marinobacter nanhaiticus，MNL）的新型脂肪酶。纯化后的 MNL 在 10-30°C 的温度范围和 8-10 的 pH 值范围内表现出很高的稳定性，在 20°C 和 pH 值为 8 时表现出最佳活性。MNL 对中等链的底物有偏好，以对硝基苯癸酸酯为底物，其最佳活性为 3768.6 U/mg 。值得注意的是，MNL 在离子型表面活性剂的作用下显示出更强的酶活性，并在蛋白酶的作用下显示出显著的弹性。洗涤性能分析进一步表明，MNL 在去除织物上的油性污渍方面具有很高的能力。总之，这些结果表明，MNL 有望成为洗衣粉配方中的重要成分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Surfactants and Detergents 工程技术-工程：化工

CiteScore

3.80

自引率

6.20%

发文量

审稿时长

4 months

期刊介绍： Journal of Surfactants and Detergents, a journal of the American Oil Chemists’ Society (AOCS) publishes scientific contributions in the surfactants and detergents area. This includes the basic and applied science of petrochemical and oleochemical surfactants, the development and performance of surfactants in all applications, as well as the development and manufacture of detergent ingredients and their formulation into finished products.