Exosomes Derived from Irradiated-Prostate Cancer Cells Promote Cancer Progression

IF 1.6 4区 医学 Q4 ENGINEERING, BIOMEDICAL
Chien‑Chih Ke, Chikondi Jassi, Chih-Hung Chuang, Chiung-Yuan Ko, Shu-Pin Huang, Shih-Hsun Kuo, Chia-Yang Li, Ya‑Ju Hsieh
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引用次数: 0

Abstract

Purpose

Radiotherapy (RT) is a commonly employed therapeutic strategy for the treatment of localized cancers, including prostate cancer (PCa). Despite significant advancements in radiotherapy technology over recent years, high recurrence and metastasis of PCa after RT remain critical challenges. Various mechanisms have been implicated in how cancer evades radiotherapy, and exosomes, a type of extracellular vesicles (EVs) has recently been identified as one of the contributing factors. This study aimed to investigate whether exosomes derived from irradiated PCa cells are involved in the cancer progression and to identify possible key factor in this process.

Methods

Exosomes were isolated from irradiated or non-irradiated PCa cell lines (designated as Rad-Exo or Exo) and characterized by specific marker expression, morphology and size. PCa cells treated with Rad-Exo or Exo were analyzed for the effects of proliferation, specific gene expression, migration and cancer stem cell property. Differential protein expression in Rad-Exo and Exo were carried out by mass spectrometry.

Results

Results showed that, compared to Exo, Rad-Exo treatment inhibited cell proliferation but significantly promoted migration and elevated the expression of genes related to epithelial to mesenchymal transition. Additionally, cells treated with Rad-Exo showed increased expression of genes related to cancer stem cells. Mass spectrometry identified POTEE as more abundant within Rad-Exo then in Exo, and its expression was confirmed to be elevated in PCa cells following irradiation. Furthermore, POTEE expression increased in cells after Rad-Exo treatment.

Conclusion

This study suggests that exosomes derived from irradiated PCa cells may function as a driver of cancer progression, including recurrent or metastatic cancer. Also, exosomal POTEE may serve as a potential target for future therapeutic or diagnostic investigations in prostate cancer.

Abstract Image

从经辐照的前列腺癌细胞中提取的外泌体促进癌症进展
目的 放射治疗(RT)是治疗包括前列腺癌(PCa)在内的局部癌症的常用治疗策略。尽管近年来放疗技术取得了重大进展,但 PCa 在放疗后的高复发率和转移率仍然是严峻的挑战。癌症逃避放疗的机制有多种,而外泌体(一种细胞外囊泡(EVs))最近被认为是其中的一个因素。本研究旨在探讨从接受过辐照的PCa细胞中提取的外泌体是否参与了癌症的进展,并找出这一过程中可能存在的关键因素。方法从接受过辐照或未接受过辐照的PCa细胞系(命名为Rad-Exo或Exo)中分离出外泌体,并通过特定标记物的表达、形态和大小对其进行表征。分析了用 Rad-Exo 或 Exo 处理的 PCa 细胞对增殖、特定基因表达、迁移和癌症干细胞特性的影响。结果表明,与 Exo 相比,Rad-Exo 处理抑制了细胞增殖,但显著促进了迁移,并提高了上皮细胞向间充质转化相关基因的表达。此外,经 Rad-Exo 处理的细胞显示癌症干细胞相关基因的表达增加。质谱分析发现,POTEE在Rad-Exo中的含量比在Exo中更高,并证实其在PCa细胞中的表达在照射后升高。结论这项研究表明,从辐照过的 PCa 细胞中提取的外泌体可能是癌症(包括复发性或转移性癌症)进展的驱动因素。此外,外泌体 POTEE 可能成为未来前列腺癌治疗或诊断研究的潜在靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.30
自引率
5.00%
发文量
81
审稿时长
3 months
期刊介绍: The purpose of Journal of Medical and Biological Engineering, JMBE, is committed to encouraging and providing the standard of biomedical engineering. The journal is devoted to publishing papers related to clinical engineering, biomedical signals, medical imaging, bio-informatics, tissue engineering, and so on. Other than the above articles, any contributions regarding hot issues and technological developments that help reach the purpose are also included.
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