Selective detection of ketamine in human urine samples by electrochemiluminescence sensor based on conductive molecular imprinted polypyrrole: A hydrogen peroxide-free electrochemiluminescence assay

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-08-14 DOI:10.1002/jccs.202400151

Farzane Ahmadi, Naader Alizadeh

{"title":"Selective detection of ketamine in human urine samples by electrochemiluminescence sensor based on conductive molecular imprinted polypyrrole: A hydrogen peroxide-free electrochemiluminescence assay","authors":"Farzane Ahmadi, Naader Alizadeh","doi":"10.1002/jccs.202400151","DOIUrl":null,"url":null,"abstract":"<p>A new sensor was fabricated for the determination of ketamine (Ket) in urine samples based on enhanced electrochemiluminescence (EECL) behavior of luminol on a carbon paste electrode (CPE) modified with the conductive molecularly imprinted polymer (CMIP) film. The FESEM technique was applied to demonstrate the rod shape morphology of CMIP. Unlike the common luminol system, EECL signal of luminol no need to addition of hydrogen peroxide and it might find application in efforts to avoid instability of hydrogen peroxide or its interference with the analytes of interest. The EECL emission has been optimized by an investigation of the applied potentials, electrochemical polymerization conditions, template and modifier concentrations, and the pH solution. Under the optimum conditions, the EECL signal depended linearly on the Ket concentration within a range from 0.1 to 1.25 μM with a limit of detection (LOD) of 0.08 μM. The sensor exhibited very good recovery percentages ranging from 92% to 101% in urine samples, while precisions were ≤3%. These results suggested that the proposed sensor would be applicable for analysis Ket in complex matrixes without interfering of other drugs (anesthetics) and thus has the potential to be used in clinical application.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jccs.202400151","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

A new sensor was fabricated for the determination of ketamine (Ket) in urine samples based on enhanced electrochemiluminescence (EECL) behavior of luminol on a carbon paste electrode (CPE) modified with the conductive molecularly imprinted polymer (CMIP) film. The FESEM technique was applied to demonstrate the rod shape morphology of CMIP. Unlike the common luminol system, EECL signal of luminol no need to addition of hydrogen peroxide and it might find application in efforts to avoid instability of hydrogen peroxide or its interference with the analytes of interest. The EECL emission has been optimized by an investigation of the applied potentials, electrochemical polymerization conditions, template and modifier concentrations, and the pH solution. Under the optimum conditions, the EECL signal depended linearly on the Ket concentration within a range from 0.1 to 1.25 μM with a limit of detection (LOD) of 0.08 μM. The sensor exhibited very good recovery percentages ranging from 92% to 101% in urine samples, while precisions were ≤3%. These results suggested that the proposed sensor would be applicable for analysis Ket in complex matrixes without interfering of other drugs (anesthetics) and thus has the potential to be used in clinical application.

Abstract Image

查看原文本刊更多论文

基于导电分子印迹聚吡咯的电化学发光传感器选择性检测人体尿样中的氯胺酮：一种不含过氧化氢的电化学发光检测方法

基于鲁米诺在导电分子印迹聚合物（CMIP）薄膜修饰的碳浆电极（CPE）上的增强电化学发光（EECL）行为，制备了一种用于测定尿样中氯胺酮（Ket）含量的新型传感器。应用 FESEM 技术展示了 CMIP 的棒状形态。与普通的发光酚系统不同，发光酚的 EECL 信号无需添加过氧化氢，可用于避免过氧化氢的不稳定性或其对相关分析物的干扰。通过对应用电位、电化学聚合条件、模板和改性剂浓度以及溶液 pH 值的研究，对 EECL 发射进行了优化。在最佳条件下，EECL 信号与 Ket 浓度在 0.1 至 1.25 μM 范围内呈线性关系，检测限（LOD）为 0.08 μM。该传感器在尿样中的回收率非常高，从 92% 到 101%，精确度≤3%。这些结果表明，该传感器可用于分析复杂基质中的 Ket，且不会受到其他药物（麻醉剂）的干扰，因此具有临床应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.