A community-oriented, data-driven resource to improve protocol design for cardiac modelling from human pluripotent stem cells

Abstract

Protocols for culturing cardiac cells derived from human-induced pluripotent stem cells (hiPSCs) are critical for studying mechanisms of development and disease. This study provides analysis of a community-oriented database to assess parameters used in published cardiac differentiation protocols, including a web-accessible portal for user-guided customisation of protocols. Validation studies support context-dependent roles for media composition in deriving functional maturation of hiPSC-derived cardiomyocytes.