Effect of the Nitrification Inhibitor DMPP on Blueberry Planted in Neutral Soil

Agronomy Pub Date : 2024-09-05 DOI:10.3390/agronomy14092029

Yiru Yang, Qilong Zeng, Hong Yu, Jiguang Wei, Jiafeng Jiang, Liangliang Tian

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Abstract

In order to increase nutrient input and alleviate the poor growth of blueberry (Vaccinium corymbosum L.) in neutral soil with strong nitrification, the application of nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) as an enhanced efficiency fertilizer is a strategy to reduce nitrogen (N) loss and improve N supply. However, few studies have systematically investigated the effect of DMPP application on blueberry and its soil condition in detail so far. In this study, a pot experiment was conducted to elucidate the effect of DMPP at four gradient levels including 0.5% (w/w applied-N) DMPP (DL), 1% DMPP (DM), 2% DMPP (DH), and no DMPP (CK) on the dynamics of soil mineral N (NH4+-N and NO3−-N), soil chemical properties, as well as the agronomic characteristics and physiological indexes of blueberry plants in the neutral soil–blueberry system. The addition of DMPP significantly increased the retention of soil ammonium nitrogen and the content of total mineral nitrogen. qPCR analysis showed that DMPP inhibited the ammoxidation process mainly by reducing the abundance of the ammonia-oxidizing bacteria (AOB) amoA gene rather than the ammonia-oxidizing archaea (AOA) amoA gene. No significant inhibitory effect of DMPP was observed for the nitrite dehydrogenase gene nxrA and nitrite reductase gene nirS. Soil NH4+-N and available phosphorus content were both enhanced with the DMPP application rates both in bulk and rhizosphere soil. Applying 1% DMPP to the neutral soil for blueberry was sufficient to safely inhibit soil nitrification, not only increasing ammonium nitrogen content by 10.42% and 26.79%, but also enhancing available phosphorus content by 9.19% and 22.41% compared with CK in bulk and rhizosphere soil, respectively. Moreover, 1% DMPP addition increased the nitrogen and phosphorus concentration of blueberry leaves by 12.17% and 26.42%, respectively, compared with CK. The total branch length and the dry weight of blueberry plant were also increased by 16.8% and 33.1%, respectively. These results provide valuable agronomic information for the application of DMPP in blueberry cultivation. Fertilization applied with 1% DMPP has great economic potential to improve both nitrogen and phosphorus absorption of blueberry so as to promote the vegetative growth of blueberry.

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硝化抑制剂 DMPP 对中性土壤中种植的蓝莓的影响

为了增加蓝莓（Vaccinium corymbosum L.）在硝化作用较强的中性土壤中的养分输入并缓解其生长不良的问题，施用硝化抑制剂 3,4-二甲基吡唑磷酸盐（DMPP）作为增效肥料是减少氮（N）流失和提高氮供应的一种策略。然而，迄今为止，很少有研究系统地详细调查了施用 DMPP 对蓝莓及其土壤条件的影响。本研究通过盆栽试验，阐明了在中性土壤-蓝莓系统中，0.5%（施用量-重量）DMPP（DL）、1% DMPP（DM）、2% DMPP（DH）和无 DMPP（CK）等四个梯度水平的 DMPP 对土壤矿物氮（NH4+-N 和 NO3--N）动态、土壤化学性质以及蓝莓植株的农艺性状和生理指标的影响。qPCR分析表明，DMPP主要通过降低氨氧化细菌（AOB）amoA基因而非氨氧化古细菌（AOA）amoA基因的丰度来抑制氨氧化过程。DMPP 对亚硝酸盐脱氢酶基因 nxrA 和亚硝酸盐还原酶基因 nirS 没有明显的抑制作用。土壤中的 NH4+-N 和可利用磷含量都随着 DMPP 施用量的增加而提高，无论是在块状土壤还是根瘤土壤中。在蓝莓的中性土壤中施用 1%的 DMPP 足以安全地抑制土壤硝化，不仅能使土壤中的铵态氮含量提高 10.42% 和 26.79%，还能使土壤中的可利用磷含量提高 9.19% 和 22.41%。此外，与 CK 相比，添加 1%的 DMPP 可使蓝莓叶片的氮和磷浓度分别提高 12.17% 和 26.42%。蓝莓植株的总枝长度和干重也分别增加了 16.8% 和 33.1%。这些结果为在蓝莓栽培中应用 DMPP 提供了宝贵的农艺学信息。施用 1%的 DMPP 可提高蓝莓对氮和磷的吸收，从而促进蓝莓的无性生长，具有很大的经济潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Agronomy

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