786. ANATOMICAL DISTRIBUTION OF HUMAN PAPILLOMAVIRUS INFECTION IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA

IF 2.3 3区医学 Q3 GASTROENTEROLOGY & HEPATOLOGY

Diseases of the Esophagus Pub Date : 2024-09-02 DOI:10.1093/dote/doae057.382

Kai-Yuan Jiang, Hirotaka Ishida, Yusuke Taniyama, Chiaki Sato, Hiroshi Okamoto, Yohei Ozawa, Takashi Kamei

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Abstract

Background Human Papillomavirus (HPV) infection is a high-risk factor for many types of cancer development. The reported infectious rate in esophageal squamous cell carcinoma (ESCC) varies widely, ranging from 0% to 88.9%. It may be caused due to geographical location, sample size, and detection methods. In general, p16 expression is considered as a biomarker for HPV infection. However, the correlation between the p16 expression and HPV infectious status has been controversial. Methods Patients with ESCC underwent esophagectomy without neoadjuvant chemotherapy (NAC) from 2007 to 2021 were included. Three formalin-fixed paraffin-embedded blocks (oral side, tumor, and esophagogastric junction (EGJ)) from each surgical resected specimen were collected. p16 expression was examined by immunohistochemistry (labeling index ≥ 10% is defined as positive expression). The presence of HPV DNA was investigated by polymerase chain reaction. In addition, the anatomical distribution of HPV infection was investigated. Results A total of 158 patients with 417 samples were included. The positive expression rate of p16 was 3.6% (5/137), 19.2% (30/156) and 2.1% (3/146) in oral side, tumor, and EGJ, respectively. The HPV infectious rate was 7% (11/158), and six cases were detected in tumor site and the other 5 cases were detected in oral side or EGJ. In tumor tissue, HPV positive cases showed p16 positive, and the sensitivity and specificity for detecting HPV DNA by IHC of p16 were 100% and 84%, respectively. The HPV infection in ESCC specimens appears to be irregular. Conclusion This study is the largest sample size in Japan, demonstrating the p16 positive rate of 19.2% and HPV infectious rate of 7% in ESCC patients without NAC. IHC of p16 can be used as a screening examination for predicting HPV infection in ESCC because the false negative rate was 0%. In addition, we firstly reported the HPV infectious mapping in ESCC specimens, and it appears irregular and random.

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786.食管鳞状细胞癌中人类乳头瘤病毒感染的解剖学分布

背景人类乳头瘤病毒（HPV）感染是多种癌症发病的高危因素。据报道，食管鳞状细胞癌（ESCC）的感染率差异很大，从 0% 到 88.9% 不等。这可能是地理位置、样本大小和检测方法造成的。一般来说，p16表达被认为是HPV感染的生物标志物。然而，p16表达与HPV感染状态之间的相关性一直存在争议。方法纳入 2007 年至 2021 年期间接受食管切除术但未接受新辅助化疗（NAC）的 ESCC 患者。收集每份手术切除标本的三个福尔马林固定石蜡包埋块（口腔侧、肿瘤和食管胃交界处（EGJ）），用免疫组化法检测 p16 表达（标记指数≥10% 定义为阳性表达）。聚合酶链反应检测是否存在 HPV DNA。此外，还调查了 HPV 感染的解剖分布情况。结果共纳入 158 名患者，417 份样本。口腔侧、肿瘤和 EGJ 中 p16 阳性表达率分别为 3.6%（5/137）、19.2%（30/156）和 2.1%（3/146）。HPV感染率为7%（11/158），其中6例在肿瘤部位检测到，另外5例在口腔侧或EGJ检测到。在肿瘤组织中，HPV 阳性病例显示 p16 阳性，通过 p16 IHC 检测 HPV DNA 的敏感性和特异性分别为 100%和 84%。ESCC 标本中的 HPV 感染似乎是不规则的。结论本研究是日本样本量最大的一项研究，结果显示，在无 NAC 的 ESCC 患者中，p16 阳性率为 19.2%，HPV 感染率为 7%。由于假阴性率为 0%，因此 p16 的 IHC 可用作预测 ESCC 中 HPV 感染的筛查检查。此外，我们首次报道了ESCC标本中的HPV感染图谱，它显得不规则且随机。

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