Sarah Friedrich, Mattia De Vivo, Christian Ulrich Baden, Michael E. Grevé, Christian Maus, Thomas Udelhoven, Henrik Krehenwinkel
{"title":"The quantity of deposited environmental DNA in plant–arthropod interactions depends on taxon, abundance and interaction time","authors":"Sarah Friedrich, Mattia De Vivo, Christian Ulrich Baden, Michael E. Grevé, Christian Maus, Thomas Udelhoven, Henrik Krehenwinkel","doi":"10.1111/een.13372","DOIUrl":null,"url":null,"abstract":"<jats:list> <jats:list-item>Environmental DNA (eDNA) metabarcoding holds great promise as a simple and efficient tool to detect plant–insect interactions. However, the influence of both abiotic and biotic factors on eDNA deposition in these interactions is not well understood. It especially remains to be tested how much eDNA abundances reflect the frequency or intensity of interactions between insects and plants.</jats:list-item> <jats:list-item>We experimentally analysed the quantitative deposition of eDNA from three insect species (the southern green stink bug <jats:italic>Nezara viridula,</jats:italic> the mustard beetle <jats:italic>Phaedon cochleariae</jats:italic> and the fall armyworm <jats:italic>Spodoptera frugiperda</jats:italic>) on leaves of two host plants of varying attractiveness: savoy cabbage (<jats:italic>Brassica oleracea</jats:italic> var. <jats:italic>sabauda</jats:italic>, preferred host) and tomato (<jats:italic>Solanum lycopersicum</jats:italic>, less preferred host). We tested for the effects of insect taxonomy, host plant, exposure time and abundances of interacting insects on the plant material.</jats:list-item> <jats:list-item>Our data show a clear quantitative ecological signal in eDNA deposition. Insect abundance and interaction time are reflected in the amount of deposited eDNA. Moreover, significantly more eDNA was deposited on cabbage, the preferred host plant. Besides these ecological drivers, a very strong taxonomic eDNA deposition bias between different insect taxa was observed.</jats:list-item> <jats:list-item>eDNA detection is strongly influenced by the ecological interactions of the targeted taxa, highlighting the utility of the tool to specifically detect trophic interactions between plants and arthropods. However, taxonomic biases in eDNA deposition preclude comparative analyses of interactions at the community level.</jats:list-item> </jats:list>","PeriodicalId":50557,"journal":{"name":"Ecological Entomology","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ecological Entomology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/een.13372","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Environmental DNA (eDNA) metabarcoding holds great promise as a simple and efficient tool to detect plant–insect interactions. However, the influence of both abiotic and biotic factors on eDNA deposition in these interactions is not well understood. It especially remains to be tested how much eDNA abundances reflect the frequency or intensity of interactions between insects and plants.We experimentally analysed the quantitative deposition of eDNA from three insect species (the southern green stink bug Nezara viridula, the mustard beetle Phaedon cochleariae and the fall armyworm Spodoptera frugiperda) on leaves of two host plants of varying attractiveness: savoy cabbage (Brassica oleracea var. sabauda, preferred host) and tomato (Solanum lycopersicum, less preferred host). We tested for the effects of insect taxonomy, host plant, exposure time and abundances of interacting insects on the plant material.Our data show a clear quantitative ecological signal in eDNA deposition. Insect abundance and interaction time are reflected in the amount of deposited eDNA. Moreover, significantly more eDNA was deposited on cabbage, the preferred host plant. Besides these ecological drivers, a very strong taxonomic eDNA deposition bias between different insect taxa was observed.eDNA detection is strongly influenced by the ecological interactions of the targeted taxa, highlighting the utility of the tool to specifically detect trophic interactions between plants and arthropods. However, taxonomic biases in eDNA deposition preclude comparative analyses of interactions at the community level.
期刊介绍:
Ecological Entomology publishes top-quality original research on the ecology of insects and related invertebrate taxa. Our aim is to publish papers that will be of considerable interest to the wide community of ecologists who are motivated by ecological or evolutionary theory. The suitability of a manuscript will usually be assessed within 5 days.
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