The transcription factor RUNT-like regulates pupal cuticle development via promoting a pupal cuticle protein transcription

IF 4.5 2区生物学 Q1 Agricultural and Biological Sciences

PLoS Genetics Pub Date : 2024-09-12 DOI:10.1371/journal.pgen.1011393

Ke-Yan Jin, Xiao-Pei Wang, Yu-Qin Di, Yu-Meng Zhao, Jin-Xing Wang, Xiao-Fan Zhao

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引用次数: 0

Abstract

Holometabolous insects undergo morphological remodeling from larvae to pupae and to adults with typical changes in the cuticle; however, the mechanism is unclear. Using the lepidopteran agricultural insect Helicoverpa armigera, cotton bollworm, as a model, we revealed that the transcription factor RUNT-like (encoded by Runt-like) regulates the development of the pupal cuticle via promoting a pupal cuticle protein gene (HaPcp) expression. The HaPcp was highly expressed in the epidermis and wing during metamorphosis and was found being involved in pupal cuticle development by RNA interference (RNAi) analysis in larvae. Runt-like was also strongly upregulated in the epidermis and wing during metamorphosis. Knockdown of Runt-like produced similar phenomena, a failure of abdomen yellow envelope and wing formation, to those following HaPcp knockdown. The insect molting hormone 20-hydroxyecdysonen (20E) upregulated HaPcp transcription via RUNT-like. 20E upregulated Runt-like transcription via nuclear receptor EcR and the transcription factor FOXO. Together, RUNT-like and HaPCP are involved in pupal cuticle development during metamorphosis under 20E regulation.

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转录因子RUNT-like通过促进蛹的角质层蛋白转录调控蛹的角质层发育

全代谢昆虫从幼虫到蛹再到成虫都会经历形态重塑，其角质层会发生典型的变化，但其机制尚不清楚。我们以鳞翅目农业昆虫棉铃虫（Helicoverpa armigera）为模型，揭示了转录因子 RUNT-like（由 Runt-like 编码）通过促进蛹角质层蛋白基因（HaPcp）的表达来调控蛹角质层的发育。在幼虫的变态过程中，HaPcp在表皮和翅膀中高度表达，通过RNA干扰（RNAi）分析发现，HaPcp参与了蛹角质层的发育。Runt-like 在变态过程中也在表皮和翅膀中强烈上调。敲除 Runt-like 会产生与敲除 HaPcp 类似的现象，即腹部黄色包膜和翅膀无法形成。昆虫蜕皮激素 20-羟基蜕皮素（20E）通过 RUNT-like 上调 HaPcp 的转录。20E 通过核受体 EcR 和转录因子 FOXO 上调 Runt-like 的转录。在 20E 的调控下，RUNT-like 和 HaPCP 共同参与了蛹变态过程中的角质层发育。

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来源期刊

PLoS Genetics 生物-遗传学

CiteScore

8.10

自引率

2.20%

发文量

438

审稿时长

1 months

期刊介绍： PLOS Genetics is run by an international Editorial Board, headed by the Editors-in-Chief, Greg Barsh (HudsonAlpha Institute of Biotechnology, and Stanford University School of Medicine) and Greg Copenhaver (The University of North Carolina at Chapel Hill). Articles published in PLOS Genetics are archived in PubMed Central and cited in PubMed.