{"title":"The transcription factor RUNT-like regulates pupal cuticle development via promoting a pupal cuticle protein transcription","authors":"Ke-Yan Jin, Xiao-Pei Wang, Yu-Qin Di, Yu-Meng Zhao, Jin-Xing Wang, Xiao-Fan Zhao","doi":"10.1371/journal.pgen.1011393","DOIUrl":null,"url":null,"abstract":"Holometabolous insects undergo morphological remodeling from larvae to pupae and to adults with typical changes in the cuticle; however, the mechanism is unclear. Using the lepidopteran agricultural insect <jats:italic>Helicoverpa armigera</jats:italic>, cotton bollworm, as a model, we revealed that the transcription factor RUNT-like (encoded by <jats:italic>Runt-like</jats:italic>) regulates the development of the pupal cuticle via promoting a pupal cuticle protein gene (<jats:italic>HaPcp</jats:italic>) expression. The <jats:italic>HaPcp</jats:italic> was highly expressed in the epidermis and wing during metamorphosis and was found being involved in pupal cuticle development by RNA interference (RNAi) analysis in larvae. <jats:italic>Runt-like</jats:italic> was also strongly upregulated in the epidermis and wing during metamorphosis. Knockdown of <jats:italic>Runt-like</jats:italic> produced similar phenomena, a failure of abdomen yellow envelope and wing formation, to those following <jats:italic>HaPcp</jats:italic> knockdown. The insect molting hormone 20-hydroxyecdysonen (20E) upregulated <jats:italic>HaPcp</jats:italic> transcription via RUNT-like. 20E upregulated <jats:italic>Runt-like</jats:italic> transcription via nuclear receptor EcR and the transcription factor FOXO. Together, RUNT-like and HaPCP are involved in pupal cuticle development during metamorphosis under 20E regulation.","PeriodicalId":20266,"journal":{"name":"PLoS Genetics","volume":null,"pages":null},"PeriodicalIF":4.5000,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"PLoS Genetics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1371/journal.pgen.1011393","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
Abstract
Holometabolous insects undergo morphological remodeling from larvae to pupae and to adults with typical changes in the cuticle; however, the mechanism is unclear. Using the lepidopteran agricultural insect Helicoverpa armigera, cotton bollworm, as a model, we revealed that the transcription factor RUNT-like (encoded by Runt-like) regulates the development of the pupal cuticle via promoting a pupal cuticle protein gene (HaPcp) expression. The HaPcp was highly expressed in the epidermis and wing during metamorphosis and was found being involved in pupal cuticle development by RNA interference (RNAi) analysis in larvae. Runt-like was also strongly upregulated in the epidermis and wing during metamorphosis. Knockdown of Runt-like produced similar phenomena, a failure of abdomen yellow envelope and wing formation, to those following HaPcp knockdown. The insect molting hormone 20-hydroxyecdysonen (20E) upregulated HaPcp transcription via RUNT-like. 20E upregulated Runt-like transcription via nuclear receptor EcR and the transcription factor FOXO. Together, RUNT-like and HaPCP are involved in pupal cuticle development during metamorphosis under 20E regulation.
期刊介绍:
PLOS Genetics is run by an international Editorial Board, headed by the Editors-in-Chief, Greg Barsh (HudsonAlpha Institute of Biotechnology, and Stanford University School of Medicine) and Greg Copenhaver (The University of North Carolina at Chapel Hill).
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