Role of male gonad-enriched microRNAs in sperm production in C. elegans.

IF 3.3 3区生物学

Genetics Pub Date : 2024-09-11 DOI:10.1093/genetics/iyae147

Lu Lu,Allison L Abbott

{"title":"Role of male gonad-enriched microRNAs in sperm production in C. elegans.","authors":"Lu Lu,Allison L Abbott","doi":"10.1093/genetics/iyae147","DOIUrl":null,"url":null,"abstract":"Germ cell development and gamete production in animals require small RNA pathways. While studies indicate that microRNAs (miRNAs) are necessary for normal sperm production and function, the specific roles for individual miRNAs are largely unknown. Here, we use small RNA sequencing of dissected gonads and functional analysis of new loss of function alleles to identify functions for miRNAs in the control of fecundity and sperm production in Caenorhabditis elegans (C. elegans) males and hermaphrodites. We describe a set of 29 male gonad-enriched miRNAs and identify a set of individual miRNAs (mir-58.1 and mir-235) and a miRNA cluster (mir-4807-4810.1) that are required for optimal sperm production at 20°C and a set of miRNAs (mir-49, mir-57, mir-83, mir-261, and mir-357/358) that are required for sperm production at 25°C. We observed defects in meiotic progression in mutants missing mir-58.1, mir-83, mir-235, and mir-4807-4810.1, which may contribute to the observed defects in sperm production. Further, analysis of multiple mutants of these miRNAs suggested genetic interactions between these miRNAs. This study provides insights on the regulatory roles of miRNAs that promote optimal sperm production and fecundity in males and hermaphrodites.","PeriodicalId":12706,"journal":{"name":"Genetics","volume":null,"pages":null},"PeriodicalIF":3.3000,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/genetics/iyae147","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Germ cell development and gamete production in animals require small RNA pathways. While studies indicate that microRNAs (miRNAs) are necessary for normal sperm production and function, the specific roles for individual miRNAs are largely unknown. Here, we use small RNA sequencing of dissected gonads and functional analysis of new loss of function alleles to identify functions for miRNAs in the control of fecundity and sperm production in Caenorhabditis elegans (C. elegans) males and hermaphrodites. We describe a set of 29 male gonad-enriched miRNAs and identify a set of individual miRNAs (mir-58.1 and mir-235) and a miRNA cluster (mir-4807-4810.1) that are required for optimal sperm production at 20°C and a set of miRNAs (mir-49, mir-57, mir-83, mir-261, and mir-357/358) that are required for sperm production at 25°C. We observed defects in meiotic progression in mutants missing mir-58.1, mir-83, mir-235, and mir-4807-4810.1, which may contribute to the observed defects in sperm production. Further, analysis of multiple mutants of these miRNAs suggested genetic interactions between these miRNAs. This study provides insights on the regulatory roles of miRNAs that promote optimal sperm production and fecundity in males and hermaphrodites.

查看原文本刊更多论文

雄性性腺富集的 microRNA 在秀丽隐杆线虫精子生成中的作用

动物生殖细胞的发育和配子的产生需要小 RNA 途径。虽然研究表明微小 RNA（miRNA）是正常精子生成和功能所必需的，但个体 miRNA 的具体作用在很大程度上是未知的。在这里，我们利用对解剖性腺的小 RNA 测序和对新的功能缺失等位基因的功能分析，来确定 miRNA 在控制秀丽隐杆线虫（C. elegans）雄性和雌雄同体的繁殖力和精子生成中的功能。我们描述了一组 29 个雄性性腺富集的 miRNAs，并鉴定出一组单个 miRNAs（mir-58.1 和 mir-235）和一个 miRNA 簇（mir-4807-4810.1），这些 miRNAs 是 20°C 最佳精子生产所必需的，以及一组 miRNAs（mir-49、mir-57、mir-83、mir-261 和 mir-357/358），这些 miRNAs 是 25°C 精子生产所必需的。我们观察到缺失 mir-58.1、mir-83、mir-235 和 mir-4807-4810.1 的突变体在减数分裂过程中存在缺陷，这可能是造成所观察到的精子生成缺陷的原因。此外，对这些 miRNAs 多个突变体的分析表明，这些 miRNAs 之间存在遗传相互作用。这项研究深入揭示了促进雄性和雌雄同体动物精子生产和繁殖力优化的 miRNA 的调控作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Genetics 生物-遗传学

CiteScore

6.20

自引率

6.10%

发文量

177

期刊介绍： GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.