Optimization of Bacillus licheniformis’ nutrient system and oilfield trial for MEOR

Dingyu Kang, Duo Sun, Zixun Yao, Zhongzhi Zhang, Zhiyong Zhang
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Abstract

The efficacy of microbial-enhanced oil recovery (MEOR) is contingent upon the performance of oil recovery functional bacteria. The optimization of the nutrient system for oil recovery functional bacteria can enhance their performance. In this study, the nutrient systems of the strains underwent screening and optimization procedures, utilizing single-factor combined with response surface methodology. Subsequently, field trials were conducted to assess the efficacy of MEOR. The results indicated that the carbon source in the nutrient system was sucrose, the nitrogen source was NaNO3, and the phosphorus source was a combination of KH2PO4 and Na2HPO4 (in a 1:1 ratio). The combination of the results from the single-factor experiments and the response surface experiment led to the following formulation for the strain F-T nutrient system (g/L): sucrose 10.0, NaNO3 2.0, KH2PO4 1.5, Na2HPO4 1.5, CaCl2 0.12, FeSO4 0.12, MgSO4·7H2O 0.2, Na2MoO4 0.08, yeast powder 1.0, and pH 7.2-7.5. The optimized F-T bacterium has the potential to spread oil up to a diameter of 40.50 mm. The results of the MEOR field trial indicated a 2.95 × 10 t increase in oil production, with a success rate of 83.87%. Following the implementation of MEOR, production levels were observed to decline to levels comparable to those observed prior to the intervention. This indicates that continued MEOR field applications may be necessary to further enhance crude oil recovery.
地衣芽孢杆菌营养系统优化和油田 MEOR 试验
微生物强化采油(MEOR)的效果取决于采油功能菌的表现。优化采油功能菌的营养系统可以提高其性能。在本研究中,利用单因素结合响应面方法,对菌株的营养系统进行了筛选和优化。随后进行了实地试验,以评估 MEOR 的功效。结果表明,营养系统中的碳源为蔗糖,氮源为 NaNO3,磷源为 KH2PO4 和 Na2HPO4 的组合(比例为 1:1)。综合单因素实验和响应面实验的结果,菌株 F-T 营养系统的配方如下(克/升):蔗糖 10.0、NaNO3 2.0、KH2PO4 1.5、Na2HPO4 1.5、CaCl2 0.12、FeSO4 0.12、MgSO4-7H2O 0.2、Na2MoO4 0.08、酵母粉 1.0、pH 值 7.2-7.5。优化后的 F-T 细菌具有将油扩散到直径 40.50 毫米的潜力。MEOR 现场试验结果表明,产油量增加了 2.95 × 10 t,成功率为 83.87%。实施 MEOR 后,观察到产量水平下降到与干预前相当的水平。这表明,为了进一步提高原油采收率,可能有必要继续进行 MEOR 现场应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
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