Morphological and molecular characters of two Helicotylenchus species from South Africa and relationship of selected soil parameters with H. pseudorobustus

IF 1.4 4区 生物学 Q3 BIOLOGY
Ebrahim Shokoohi, Candice van Rensburg, Zafar Handoo, Peter Masoko
{"title":"Morphological and molecular characters of two Helicotylenchus species from South Africa and relationship of selected soil parameters with H. pseudorobustus","authors":"Ebrahim Shokoohi, Candice van Rensburg, Zafar Handoo, Peter Masoko","doi":"10.1007/s11756-024-01785-4","DOIUrl":null,"url":null,"abstract":"<p>During a survey of plant-parasitic nematodes in South Africa's Limpopo Province, two species of <i>Helicotylenchus</i> were identified, namely <i>H. dihystera</i> and <i>H. pseudorobustus</i>. The morphological and molecular characteristics of these species were found to be consistent with those of the known species. A phylogenetic analysis of <i>Helicotylenchus</i> populations based on 28S rDNA was conducted, and it was found that the <i>H. dihystera</i> identified in this study belonged to the same group as other <i>H. dihystera</i> specimens with a 1.00 posterior probability support. Moreover, phylogenetic analysis of <i>H. pseudorobustus</i> based on 18S rDNA placed the test population close to other <i>H. pseudorobustus</i> specimens with 0.97 posterior probability. Scanning electron microscopy (SEM) for <i>Helicotylenchus</i> species also revealed noticeable dissimilarities in the labial disc and lateral field of the tail region between the two species from the present study, including <i>H. pseudorobustus</i>, and <i>H. dihystera</i>. The redundancy analysis (RDA) showed that <i>H. pseudorobustus</i> had a correlation with pH and clay of the soil. In conclusion, despite the challenges associated with identifying <i>Helicotylenchus</i> species, SEM and rDNA markers can be considered as highly effective tools to distinguish the species correctly and accurately.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biologia","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11756-024-01785-4","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

During a survey of plant-parasitic nematodes in South Africa's Limpopo Province, two species of Helicotylenchus were identified, namely H. dihystera and H. pseudorobustus. The morphological and molecular characteristics of these species were found to be consistent with those of the known species. A phylogenetic analysis of Helicotylenchus populations based on 28S rDNA was conducted, and it was found that the H. dihystera identified in this study belonged to the same group as other H. dihystera specimens with a 1.00 posterior probability support. Moreover, phylogenetic analysis of H. pseudorobustus based on 18S rDNA placed the test population close to other H. pseudorobustus specimens with 0.97 posterior probability. Scanning electron microscopy (SEM) for Helicotylenchus species also revealed noticeable dissimilarities in the labial disc and lateral field of the tail region between the two species from the present study, including H. pseudorobustus, and H. dihystera. The redundancy analysis (RDA) showed that H. pseudorobustus had a correlation with pH and clay of the soil. In conclusion, despite the challenges associated with identifying Helicotylenchus species, SEM and rDNA markers can be considered as highly effective tools to distinguish the species correctly and accurately.

Abstract Image

南非两种斜纹盘尾丝虫的形态和分子特征以及某些土壤参数与假盘尾丝虫的关系
在对南非林波波省的植物寄生线虫进行调查期间,发现了两种 Helicotylenchus,即 H. dihystera 和 H. pseudorobustus。这些物种的形态和分子特征与已知物种一致。根据28S rDNA对Helicotylenchus种群进行了系统进化分析,发现本研究中鉴定出的H. dihystera与其他H. dihystera标本属于同一类群,后验概率支持率为1.00。此外,基于 18S rDNA 的 H. pseudorobustus 系统进化分析表明,试验种群与其他 H. pseudorobustus 标本接近,后验概率为 0.97。扫描电子显微镜(SEM)对Helicotylenchus物种的观察还发现,本研究中的两个物种(包括H. pseudorobustus和H. dihystera)的唇盘和尾部外侧区域存在明显差异。冗余分析(RDA)显示,伪尾柱虫与土壤的酸碱度和粘度有关。总之,尽管在识别斜纹夜蛾物种方面存在挑战,但扫描电镜和rDNA标记可被视为正确、准确区分物种的高效工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Biologia
Biologia 生物-生物学
CiteScore
3.30
自引率
6.70%
发文量
290
审稿时长
6 months
期刊介绍: Established in 1946, Biologia publishes high-quality research papers in the fields of microbial, plant and animal sciences. Microbial sciences papers span all aspects of Bacteria, Archaea and microbial Eucarya including biochemistry, cellular and molecular biology, genomics, proteomics and bioinformatics. Plant sciences topics include fundamental research in taxonomy, geobotany, genetics and all fields of experimental botany including cellular, whole-plant and community physiology. Zoology coverage includes animal systematics and taxonomy, morphology, ecology and physiology from cellular to molecular level.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信