Identification and prediction of functions for drought-responsive genes in sugar beet (Beta vulgaris L.)

IF 1.4 4区 生物学 Q3 BIOLOGY
Chunlei Zou, Shanshan Zhao, Wenting Chai, Bohui Yang, Chunlai Zhang, Zhijia Gai
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Abstract

Drought is an environmental stress factor restricting the growth and productivity of plants. Sugar beet can acclimate to diverse types of abiotic stress, such as drought. Although many studies on response of sugar beet to drought stress have been conducted, the expressions of drought-responsive genes in sugar beet have rarely been comprehensively investigated. In this study, we performed a transcriptome analysis of leaf samples from water-controlled sugar beet seedlings. The clean reads were obtained and assembled into 21,749 unique genes. Among them, we identified 1,675 differentially expressed genes (DEGs). According to the findings of the Gene ontology (GO) analysis, “biological process”, “metabolic process”, “oxidation–reduction process” and “catalytic activity” were the most highly enriched GO terms. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that “biosynthesis of secondary metabolites”, “metabolic pathways”, “plant hormone signal transduction”, and “protein processing in endoplasmic reticulum” were the four most highly enriched pathways. Drought stress significantly restrained dry mass and net photosynthetic rate of sugar beet. The expression levels of genes encoding homeobox-leucine zipper protein ATHB-12, berberine bridge enzyme-like 13, auxin response factor 5, and photosystem I subunit O were significantly altered under drought stress. In addition, 36 DEGs occurred with differential alternative splicing. Our findings provided new insights into the regulatory functions of genes related to drought resistance in sugar beet.

Abstract Image

甜菜(Beta vulgaris L.)中干旱响应基因的鉴定和功能预测
干旱是一种限制植物生长和生产力的环境胁迫因素。甜菜能适应多种类型的非生物胁迫,如干旱。虽然有关甜菜对干旱胁迫响应的研究很多,但对甜菜中干旱响应基因的表达却很少有全面的研究。在本研究中,我们对控水甜菜幼苗的叶片样本进行了转录组分析。我们获得了干净的读数,并将其组装成 21,749 个独特的基因。其中,我们发现了 1,675 个差异表达基因(DEGs)。根据基因本体(Gene ontology,GO)分析结果,"生物过程"、"代谢过程"、"氧化还原过程 "和 "催化活性 "是富集程度最高的GO术语。京都基因和基因组百科全书(KEGG)分析表明,"次生代谢物的生物合成"、"代谢途径"、"植物激素信号转导 "和 "内质网蛋白质加工 "是富集程度最高的四个途径。干旱胁迫明显抑制了甜菜的干重和净光合速率。在干旱胁迫下,编码同源框-亮氨酸拉链蛋白 ATHB-12、小檗碱桥酶样 13、辅助因子 5 和光合系统 I 亚基 O 的基因的表达水平发生了显著变化。此外,有 36 个 DEGs 发生了不同的替代剪接。我们的研究结果为了解甜菜抗旱相关基因的调控功能提供了新的视角。
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来源期刊
Biologia
Biologia 生物-生物学
CiteScore
3.30
自引率
6.70%
发文量
290
审稿时长
6 months
期刊介绍: Established in 1946, Biologia publishes high-quality research papers in the fields of microbial, plant and animal sciences. Microbial sciences papers span all aspects of Bacteria, Archaea and microbial Eucarya including biochemistry, cellular and molecular biology, genomics, proteomics and bioinformatics. Plant sciences topics include fundamental research in taxonomy, geobotany, genetics and all fields of experimental botany including cellular, whole-plant and community physiology. Zoology coverage includes animal systematics and taxonomy, morphology, ecology and physiology from cellular to molecular level.
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