A Simple Method for the Preparation and Characterization of Nanostructured Lipid Carriers (NLC) Formulations for Cutaneous Use

Abstract

Nanostructured lipid carriers (NLC) are promising nanocarriers for improving the delivery of drugs and cosmetics. This laboratory study aims to familiarize Master’s level students from the areas of Pharmaceutical Sciences, Biology and Biomedical Engineering with the applications of nanotechnology in healthcare, pharmaceuticals, and cosmetics. In particular, it provides students with essential knowledge on the preparation and characterization of formulations of lipid nanoparticles, namely, NLC, for cutaneous use. Ibuprofen has been used as a lipophilic drug model. The experimental protocol included two class sessions of 120 min each. Collaboration in groups of three students facilitated a comprehensive exploration of the learning methods. The students prepared NLC loaded with a model lipophilic drug (ibuprofen) with the aim of incorporating it into a hydrogel to facilitate their cutaneous use. The ibuprofen-loaded NLC were characterized in terms of particle size, polydispersity index (PDI), zeta potential (ZP) and encapsulation efficiency (EE). Rheological characterization of the prepared hydrogel containing ibuprofen-loaded NLC was then carried out. Both laboratory sessions were successful, demonstrating a strong understanding of the concepts taught. Student scores ranged from 93% to 100%, reflecting a high level of comprehension.