A Novel General Chemistry Experiment for Freshmen: Transgenic Soybean Detection Based on Microfluidic Molecular Fluorescence Spectroscopy Analysis

IF 2.5 3区教育学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Journal of Chemical Education Pub Date : 2024-09-05 DOI:10.1021/acs.jchemed.4c00668

Pintao Li, Min Gu, Ghazala Ashraf, Huiwen Xiong, Fei Cun, Xuting Chen, Jilie Kong, Xueen Fang

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Abstract

In this experiment, we present a microfluidic-based molecular fluorescence spectroscopy method for analyzing nucleic acids to identify transgenic soybeans. This method is integrated into a General Chemistry Experiment course tailored for freshmen. The primary goals of this course are to deepen student’s understanding of some important knowledge points of general chemistry, introduce students to experimental techniques at the micro- and nanoscale, help students understand the principles of molecular fluorescence spectroscopy and enzyme reaction kinetics, elucidate the relationship between chemistry and its practical applications, stimulate their interest in chemistry, and provide multidisciplinary perspectives and thinking. Over the period from 2021 to 2023, more than 180 freshmen enrolled in this course, and over 30 universities in China have initiated the advancement of this course. Most students successfully completed the experiment, achieving high completion rate and promising results. Participating students improved their practical skills and the ability to work collaboratively in a laboratory setting, which led to numerous innovative ideas and insights in related areas. Positive feedback from the students confirmed that the predetermined learning objectives were successfully achieved.

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面向高一新生的新型普通化学实验：基于微流控分子荧光光谱分析的转基因大豆检测

在本实验中，我们介绍了一种基于微流体的分子荧光光谱法，用于分析核酸以鉴定转基因大豆。该方法被整合到为大一新生量身定制的普通化学实验课程中。该课程的主要目标是加深学生对普通化学一些重要知识点的理解，向学生介绍微米和纳米尺度的实验技术，帮助学生理解分子荧光光谱学和酶反应动力学的原理，阐明化学与实际应用的关系，激发学生对化学的兴趣，提供多学科的视野和思维。从2021年到2023年，共有180多名大一新生选修了这门课程，全国已有30多所高校启动了这门课程的推进工作。大部分学生顺利完成了实验，取得了较高的完成率和可喜的成果。参加实验的学生提高了实践技能和在实验室环境中协同工作的能力，在相关领域提出了许多创新想法和见解。学生们的积极反馈证实，预定的学习目标已成功实现。

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来源期刊

Journal of Chemical Education 化学-化学综合

CiteScore

5.60

自引率

50.00%

发文量

465

审稿时长

6.5 months

期刊介绍： The Journal of Chemical Education is the official journal of the Division of Chemical Education of the American Chemical Society, co-published with the American Chemical Society Publications Division. Launched in 1924, the Journal of Chemical Education is the world’s premier chemical education journal. The Journal publishes peer-reviewed articles and related information as a resource to those in the field of chemical education and to those institutions that serve them. JCE typically addresses chemical content, activities, laboratory experiments, instructional methods, and pedagogies. The Journal serves as a means of communication among people across the world who are interested in the teaching and learning of chemistry. This includes instructors of chemistry from middle school through graduate school, professional staff who support these teaching activities, as well as some scientists in commerce, industry, and government.