Pcbp1 constrains Oct4 expression in the context of pluripotency

Abstract

Oct4 is a commonly known marker of pluripotent stem cells as well as one of the key factors required for pluripotency induction. Its gene (Pou5f1) is subject to complicated regulation through distal and proximal enhancers. Noteworthy, this protein also plays an important role in primitive endoderm (PrE) specification, though the mechanisms driving its expression during this process are still unknown. Here we show that KH-domain protein Pcbp1 occupies poly(C)-sites of the Pou5f1 enhancers, but Pcbp1 knockout does not affect the Oct4 expression level in ESCs. On the contrary, Pcbp1 is essential for timely Oct4 downregulation upon differentiation signals. Residual Oct4 expression in turn leads to PrE specification, and this phenotype is reminiscent of that in ESCs constitutively expressing Oct4. Overall, our results point to Pcbp1 is a transcriptional regulator of Pou5f1, purported to synchronize Oct4 expression decline with the pluripotency network shutdown during differentiation. Oct4 being outside of this network loss its functions as factor of pluripotency and acts as PrE specifier.