O. V. Chubukova, L. R. Khakimova, R. T. Matnyazov, Z. R. Vershinina
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引用次数: 0
Abstract
Three bacteria strains of Pseudomonas sp. resistant to heavy metals were isolated from chemically contaminated soil. An analysis of the nucleotide sequences of the 16S rRNA and rpoD genes identified the Pseudomonas sp. 17 НМ strain as Pseudomonas capeferrum, and the strains of Pseudomonas sp. 65 НМ and 67 НМ were most closely related to the type strains of Pseudomonas silesiensis and Pseudomonas umsongensis, respectively. It was shown that the strains of Pseudomonas sp. 17 НМ, 65 НМ, and 67 НМ were characterized by different levels of resistance to heavy metals, with the maximum tolerable concentration (MTC) of zinc being 1 mМ for all strains, that of cadmium, 1, 1.5, and 1 mМ; of lead, 5, 5, and 4 mМ; and of nickel, 7, 9, and 7 mМ, respectively. All Pseudomonas strains can form biofilms and have the properties of PGPR bacteria. Treatment of alfalfa seeds (Medicago sativa L.) with the strains of Pseudomonas sp. 17 НМ, 65 НМ, and 67 НМ under cadmium stress increased the dry weight of alfalfa seedlings up to 40% and the content of chlorophyll a and b in the leaves by 25–33% relative to the control.
期刊介绍：
Biology Bulletin (Izvestiya Rossiiskoi Akademii Nauk – Seriya Biologicheskaya) is an interdisciplinary journal of general biology. It focuses on fundamental studies in the fields of cell biology, biochemistry, zoology, botany, physiology, and ecology. This journal publishes current materials of experimental studies and surveys on current problems in general biology. It also publishes information on scientific conferences and new books in the fields of general biology.
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