IRF9 and STAT1 as biomarkers involved in T-cell immunity in atherosclerosis

IF 2.1 4区 生物学 Q2 BIOLOGY
Wei Xie, Xiang Gao, Liang Zhao, Shifei Song, Na Li, Junming Liu
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引用次数: 0

Abstract

Atherosclerosis is a common cardiovascular disease in which the arteries are thickened due to buildup of plaque. This study aims to identify programmed cell death (PCD)-related biomarkers and explore the crucial regulatory mechanisms of atherosclerosis. Gene expression profiles of atherosclerosis and control groups from GSE20129 and GSE23746 were obtained. Necroptosis was elevated in atherosclerosis. Weighted gene co-expression network analysis (WGCNA) was conducted in GSE23746 and GSE56045 to identify PCD-related modules and to perform enrichment analysis. Two necroptosis-related genes (IRF9 and STAT1) were identified and considered as biomarkers. Enrichment analysis showed that these gene modules were mainly related to immune response regulation. In addition, single-cell RNA sequencing data from GSE159677 were obtained and the characteristic cell types of atherosclerosis were identified. A total of 11 immune cell types were identified through UMAP dimension reduction. Most immune cells were mainly enriched in plaque samples, and STAT1 and IRF9 were primarily expressed in T-cells and macrophages. Moreover, the roles of IRF9 and STAT1 were assessed and found to be significantly upregulated in atherosclerosis, which was associated with increased risk of atherosclerosis. This study provides a molecular feature of atherosclerosis, offering an important basis for further research on its pathological mechanisms and the search for new therapeutic targets.

Abstract Image

IRF9和STAT1是参与动脉粥样硬化中T细胞免疫的生物标记物
动脉粥样硬化是一种常见的心血管疾病,动脉粥样斑块的堆积会导致动脉增厚。本研究旨在确定与程序性细胞死亡(PCD)相关的生物标志物,并探索动脉粥样硬化的关键调控机制。研究人员从 GSE20129 和 GSE23746 中获得了动脉粥样硬化组和对照组的基因表达谱。动脉粥样硬化中坏死率升高。在 GSE23746 和 GSE56045 中进行了加权基因共表达网络分析(WGCNA),以确定 PCD 相关模块并进行富集分析。确定了两个坏死相关基因(IRF9 和 STAT1),并将其视为生物标志物。富集分析表明,这些基因模块主要与免疫反应调控有关。此外,还从 GSE159677 中获得了单细胞 RNA 测序数据,并确定了动脉粥样硬化的特征细胞类型。通过 UMAP 降维,共鉴定出 11 种免疫细胞类型。大多数免疫细胞主要富集在斑块样本中,STAT1 和 IRF9 主要在 T 细胞和巨噬细胞中表达。此外,研究还评估了 IRF9 和 STAT1 的作用,发现它们在动脉粥样硬化中显著上调,这与动脉粥样硬化风险的增加有关。这项研究提供了动脉粥样硬化的分子特征,为进一步研究其病理机制和寻找新的治疗靶点提供了重要依据。
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来源期刊
Journal of Biosciences
Journal of Biosciences 生物-生物学
CiteScore
5.80
自引率
0.00%
发文量
83
审稿时长
3 months
期刊介绍: The Journal of Biosciences is a quarterly journal published by the Indian Academy of Sciences, Bangalore. It covers all areas of Biology and is the premier journal in the country within its scope. It is indexed in Current Contents and other standard Biological and Medical databases. The Journal of Biosciences began in 1934 as the Proceedings of the Indian Academy of Sciences (Section B). This continued until 1978 when it was split into three parts : Proceedings-Animal Sciences, Proceedings-Plant Sciences and Proceedings-Experimental Biology. Proceedings-Experimental Biology was renamed Journal of Biosciences in 1979; and in 1991, Proceedings-Animal Sciences and Proceedings-Plant Sciences merged with it.
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