The RNAi lethal effect of a serine protease gene (TcSP3) on Tetranychus cinnabarinus and its safety evaluation

IF 5.6 1区农林科学 Q1 ENTOMOLOGY

Entomologia Generalis Pub Date : 2024-08-22 DOI:10.1127/entomologia/2024/2335

Xing-Ru Chen, Teng-Yue Rao, Guang-Ming Liu, Yi-Ni Chen, Yan-Jie Luo, Lin He, Guang-Mao Shen

引用次数: 0

Abstract

RNA interference technology has been recognized in recent years as an ideal and specific new approach for pest control. In this study, a serine protease gene TcSP3 was found specifically expressed during the egg stage of Tetranychus cinnabarinus. Treatment of dsTcSP3 caused high mortality in egg and larvae stages of T. cinnabarinus, but a lower mortality rate after treating adult mites. Persistence analysis showed that dsTcSP3 degraded rapidly after application to cowpea leaves and was essentially undetectable after 60 h. It was also safe to Neoseiulus barkeri and caused essentially no death or deformity of N. barkeri. In conclusion, silencing of the serine protease gene TcSP3 successfully impeded the growth and development process of T. cinnabarinus with less impact on the environment and predatory mite (Neoseiulus barkeri), which can be used as a new target of RNAi for T. cinnabarinus.

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丝氨酸蛋白酶基因（TcSP3）对朱砂菌的 RNAi 致死效应及其安全性评估

近年来，RNA 干扰技术已被认为是一种理想而特异的害虫控制新方法。本研究发现，丝氨酸蛋白酶基因 TcSP3 在朱砂虫卵期特异性表达。用dsTcSP3处理朱砂绢螨的卵和幼虫阶段，死亡率很高，但处理成螨之后，死亡率较低。持久性分析表明，dsTcSP3 在豇豆叶片上施用后会迅速降解，60 小时后基本上检测不到。总之，沉默丝氨酸蛋白酶基因 TcSP3 成功地阻碍了朱砂蝇的生长发育过程，对环境和捕食螨（Neoseiulus barkeri）的影响较小，可作为朱砂蝇 RNAi 的新靶标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Entomologia Generalis 生物-昆虫学

CiteScore

7.10

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Its scope covers all aspects of basic and applied research dealing with insects and more broadly with arthropods inhabiting wild, agricultural and/or urban habitats. The journal also considers research integrating various disciplines and issues within the broad field of entomology and ecology. Entomologia Generalis publishes high quality research articles on advances in knowledge on the ecology and biology of arthropods, as well as on their importance for key ecosystems services, e.g. as biological control and pollination. The journal devotes special attention to contributions providing significant advances (i) on the fundamental knowledge and on sustainable control strategies of arthropod pests (including of stored products) and vectors of diseases, (ii) on the biology and ecology of beneficial arthropods, (iii) on the spread and impact of invasive pests, and (iv) on potential side effects of pest management methods. Entomologia Generalis welcomes review articles on significant developments in the field of entomology. These are usually invited by the editorial board, but proposals may be sent to the Editor-in-Chief for preliminary assessment by the editorial board before formal submission to the journal. The journal also considers comments on papers published in Entomologia Generalis, as well as short notes on topics that are of broader interest.