Potential of the entomopathogenic fungus Metarhizium robertsii to control radicicole grape phylloxera Daktulosphaira vitifoliae

IF 5.6 1区农林科学 Q1 ENTOMOLOGY

Entomologia Generalis Pub Date : 2024-09-05 DOI:10.1127/entomologia/2024/2689

Mathilde Ponchon, Daciana Papura, Rodrigo López-Plantey, Benjamin Joubard, Manmeet Singh, Karima Rahmani, Annette Reineke, Denis Thiéry

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引用次数: 0

Abstract

Abstract: In most viticulture countries, the grape phylloxera (GP) Daktulosphaira vitifoliae (Fitch) (Hemiptera: Phylloxeridae) is controlled by planting grafted plants on rootstocks resistant to this pest. In the search for alternative protective methods, the effectiveness of Metarhizium robertsii in protecting non-grafted Vitis vinifera grapevines against radicicole GP was assessed in vitro. In the first approach, GP eggs from a single clonal lineage were infected with M. robertsii strain EF3.5(2) obtained from a French vineyard. In bioassays, three application methods (egg spraying, root spraying, and both) were assessed using fresh woody root sections of V. vinifera cv. Cabernet Sauvignon. M. robertsii pathogenic effect translated to a significant reduction of 90.0 % by the egg spraying, 70.0 % by the root spraying, and 86.1 % by spraying both in the survival probability of GP larvae and adults compared to controls, and 92.0 %, 89.0 % and 93.0 % in the reduction of successful development to the adult stage after 28 days post-treatment, respectively. In the second approach, the impact of M. robertsii’s association with non-grafted V. vinifera roots on radicicole GP establishment and symptom induction was assessed in a pot experiment. After 76 days post-GP inoculation, a remarkable 91 % reduction in damage was observed in plants co-inoculated with M. robertsii and GP, compared to those infected solely with GP. Furthermore, 100 % of M. robertsii-inoculated plants and 92.0 % of GP-M. robertsii co-inoculated plants were colonized by the fungus at the rhizosphere level. Notably, grapevine growth remained unaffected during the trial by neither M. robertsii inoculation nor GP infestation. In conclusion, these findings highlight the potential of M. robertsii for durable association and GP biocontrol in non-grafted grapevines. Future investigations are warranted under field conditions to validate and optimize the larger-scale practical application of this biocontrol strategy.

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昆虫病原真菌 Metarhizium robertsii 控制根瘤葡萄根瘤蚜 Daktulosphaira vitifoliae 的潜力

摘要：在大多数葡萄栽培国家，葡萄根瘤蚜（GP）Daktulosphaira vitifoliae (Fitch) (Hemiptera: Phylloxeridae)是通过在抗这种害虫的砧木上种植嫁接植物来控制的。为了寻找替代保护方法，我们在体外评估了 Metarhizium robertsii 在保护非嫁接葡萄藤免受 Radicicole GP 侵害方面的有效性。第一种方法是用从法国葡萄园获得的罗伯茨梅氏菌株 EF3.5(2)感染来自单一克隆系的 GP 卵。在生物测定中，使用 V. vinifera cv. Cabernet Sauvignon 的新鲜木质根切片对三种施用方法（卵喷洒、根部喷洒和两种方法）进行了评估。与对照组相比，喷洒虫卵、喷洒根部和同时喷洒三种方法分别显著降低了 90.0 %、70.0 % 和 86.1 % 的 GP 幼虫和成虫存活率，并显著降低了 92.0 %、89.0 % 和 93.0 % 的成虫成功率。第二种方法是在盆栽实验中评估 M. robertsii 与非嫁接葡萄根系的结合对根瘤菌 GP 的建立和症状诱导的影响。在接种 GP 76 天后，与只感染 GP 的植株相比，接种了罗伯茨灰霉病菌和 GP 的植株所受的损害明显减少了 91%。此外，100% 接种了罗伯茨真菌的植株和 92.0% 接种了 GP-M. 罗伯茨真菌的植株在根瘤层都有真菌定殖。值得注意的是，在试验期间，无论是接种罗伯茨真菌还是 GP 侵染都不会影响葡萄的生长。总之，这些研究结果凸显了罗伯茨真菌在非嫁接葡萄藤中持久结合和 GP 生物防治的潜力。未来有必要在田间条件下进行调查，以验证和优化这种生物防治策略在更大范围内的实际应用。

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来源期刊

Entomologia Generalis 生物-昆虫学

CiteScore

7.10

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Its scope covers all aspects of basic and applied research dealing with insects and more broadly with arthropods inhabiting wild, agricultural and/or urban habitats. The journal also considers research integrating various disciplines and issues within the broad field of entomology and ecology. Entomologia Generalis publishes high quality research articles on advances in knowledge on the ecology and biology of arthropods, as well as on their importance for key ecosystems services, e.g. as biological control and pollination. The journal devotes special attention to contributions providing significant advances (i) on the fundamental knowledge and on sustainable control strategies of arthropod pests (including of stored products) and vectors of diseases, (ii) on the biology and ecology of beneficial arthropods, (iii) on the spread and impact of invasive pests, and (iv) on potential side effects of pest management methods. Entomologia Generalis welcomes review articles on significant developments in the field of entomology. These are usually invited by the editorial board, but proposals may be sent to the Editor-in-Chief for preliminary assessment by the editorial board before formal submission to the journal. The journal also considers comments on papers published in Entomologia Generalis, as well as short notes on topics that are of broader interest.