E. Yu. Brusentsev, T. A. Rakhmanova, I. N. Rozhkova, S. V. Okotrub, V. S. Kozeneva, S. Ya. Amstislavsky
{"title":"Experimental Approaches to Controlled Diapause in Mammals","authors":"E. Yu. Brusentsev, T. A. Rakhmanova, I. N. Rozhkova, S. V. Okotrub, V. S. Kozeneva, S. Ya. Amstislavsky","doi":"10.1134/s1067413624700097","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Diapause is a coping strategy characteristic to many invertebrates and vertebrates including more than 100 mammalian species. Preserving the genetic diversity of rare and endangered diapausing species is important for maintaining ecological systems. The purpose of this work was to compare the surgical model of diapause and the pharmacological model based on injecting DL-α-difluoromethylornithine (DL-α-DFMO) into mice. Another goal was to investigate the intriguing possibility of controlling the state of diapause in vitro by exposing murine embryos to putrescine and/or DL-α-DFMO. Although the pharmacological model <i>a priori</i> seems to be attractive for applying it to other mammalian species besides mice, since it does not require surgical intervention, our results on mice demonstrated that this model is less effective compared to the traditional surgical model of diapause. Our data indicates that the effects of DL-α-DFMO on mouse embryos are mediated via its effect on the uterus, as it was not possible to maintain dormancy state in diapausing embryos in vitro by this agent. Meanwhile, in vitro exposure to putrescine facilitates the re-activation of diapausing embryos, as evidenced by the higher rate of blastocysts adhesion and the more advanced stages of ICM outgrowth compared to controls. Our results on mice presented hereby indicate that the surgical model is more reliable than DL-α-DFMO diapause model. Moreover, our results proved that putrescine is a potent tool to re-activate murine diapausing embryos in vitro; this may be considered an ecologically important issue relevant to the conservation problem.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1134/s1067413624700097","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Diapause is a coping strategy characteristic to many invertebrates and vertebrates including more than 100 mammalian species. Preserving the genetic diversity of rare and endangered diapausing species is important for maintaining ecological systems. The purpose of this work was to compare the surgical model of diapause and the pharmacological model based on injecting DL-α-difluoromethylornithine (DL-α-DFMO) into mice. Another goal was to investigate the intriguing possibility of controlling the state of diapause in vitro by exposing murine embryos to putrescine and/or DL-α-DFMO. Although the pharmacological model a priori seems to be attractive for applying it to other mammalian species besides mice, since it does not require surgical intervention, our results on mice demonstrated that this model is less effective compared to the traditional surgical model of diapause. Our data indicates that the effects of DL-α-DFMO on mouse embryos are mediated via its effect on the uterus, as it was not possible to maintain dormancy state in diapausing embryos in vitro by this agent. Meanwhile, in vitro exposure to putrescine facilitates the re-activation of diapausing embryos, as evidenced by the higher rate of blastocysts adhesion and the more advanced stages of ICM outgrowth compared to controls. Our results on mice presented hereby indicate that the surgical model is more reliable than DL-α-DFMO diapause model. Moreover, our results proved that putrescine is a potent tool to re-activate murine diapausing embryos in vitro; this may be considered an ecologically important issue relevant to the conservation problem.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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