New status of Bichromomyia subspecies (Diptera: Psychodidae: Phlebotominae) based on molecular taxonomy

IF 2.1 3区农林科学 Q1 ENTOMOLOGY

Journal of Medical Entomology Pub Date : 2024-09-10 DOI:10.1093/jme/tjae099

Yokomi N Lozano-Sardaneta, Herón Huerta, Yesenia Marquez-López, Atilano Contreras-Ramos

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Abstract

The sand fly of the genus Bichromomyia (Galati, 1995) includes 3 subspecies considered vectors of Leishmania, which share high morphological similarity. Through information from the Cytochrome Oxidase Subunit I (COI) gene, we provide complementary evidence to support that Bichromomyia olmeca olmeca, and Bichromomyia olmeca bicolor, should be raised to nominal species status. We recovered specimens of Bi. o. olmeca from Quintana Roo, Tabasco, and Oaxaca, Mexico, supply 17 new COI sequences, and also incorporate GenBank sequences for other Bichromomyia species. After a Maximum Likelihood (ML) analysis, all Bichromomyia species clustered with a bootstrap of 100%, although sequences of Bichromomyia flaviscutellata were divided into 2 clusters with an interspecific range distance of 11.16% between them, which confirm cryptic species in Brazil. The genetic distance of Bi. o. olmeca compared to related subspecies ranged between 12.59% and 14.64%. A total of 29 haplotypes (Hd = 0.987; π = 0.08783; S = 136) were recovered from the Bichromomyia sequences. Results of the TC network were consistent with the ML analysis, supporting that subspecies of Bichromomyia are genetically distinct and deserve being raised to valid species category: Bichromomyia olmeca (Vargas & Díaz-Nájera) and Bichromomyia bicolor (Fairchild & Theodor).

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基于分子分类学的 Bichromomyia 亚种新地位（双翅目：灵虫科：Phlebotominae

沙蝇属 Bichromomyia（Galati，1995 年）包括 3 个亚种，被认为是利什曼病的传播媒介，它们在形态上具有高度相似性。通过细胞色素氧化酶亚基 I（COI）基因的信息，我们提供了补充证据，支持将 Bichromomyia olmeca olmeca 和 Bichromomyia olmeca bicolor 提升到标称种的地位。我们从墨西哥金塔纳罗奥州、塔巴斯科州和瓦哈卡州采集到了 Bichromomyia olmeca olmeca 的标本，提供了 17 条新的 COI 序列，并纳入了 GenBank 中其他 Bichromomyia 物种的序列。经过最大似然法（ML）分析，所有 Bichromomyia 物种的聚类引导率均为 100%，但 Bichromomyia flaviscutellata 的序列被分为 2 个聚类，它们之间的种间距离为 11.16%，这证实了巴西的隐性物种。o. olmeca 与相关亚种的遗传距离在 12.59% 和 14.64% 之间。从 Bichromomyia 序列中总共发现了 29 个单倍型（Hd = 0.987；π = 0.08783；S = 136）。TC 网络的结果与 ML 分析结果一致，证明 Bichromomyia 亚种在遗传学上是不同的，值得将其提升为有效的物种类别：Bichromomyia olmeca (Vargas & Díaz-Nájera) 和 Bichromomyia bicolor (Fairchild & Theodor)。

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来源期刊

Journal of Medical Entomology 生物-昆虫学

CiteScore

4.60

自引率

14.30%

发文量

207

审稿时长

3-8 weeks

期刊介绍： Journal of Medical Entomology is published bimonthly in January, March, May, July, September, and November. The journal publishes reports on all phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of public health and veterinary significance. In addition to full-length research articles, the journal publishes Reviews, interpretive articles in a Forum section, Short Communications, and Letters to the Editor.