Genome-wide identification and expression analysis of MYB transcription factor family in Rosa persica

Abstract

MYB transcription factors play pivotal roles in various facets of plant morphogenesis, maturation, and essential physiological functions. Despite the essential nature of MYB family genes (MYBs) in plant biology, their functions and expression patterns in Rosa persica remain incompletely characterized. This poses an obstacle for the further identification of the functions of related genes in R. persica. To shed light on the structural and expression characteristics of MYB gene family members in R. persica, 147 RbeMYBs were identified within the R. persica genome. Subsequently, a thorough examination of their chromosomal localization, gene structure, and protein structural domains was conducted. The phylogeny of the MYB transcription factor family of R. persica was analyzed, collinearity analyses were performed with MYBs of Arabidopsis thaliana and other species, and expression profiles were obtained through RNA-Seq and qRT-PCR. This article identifies the R. persica MYB family, offers preliminary insights into its potential functions, and pinpoints six candidate genes related to pigment synthesis. This knowledge provides a foundation for future developments in R. persica flower color breeding.