{"title":"Unveiling reproductive biology, phenology, and pollen viability in Lathyrus species to enhance crop improvement","authors":"Muthusamy Shankar, Ravi Gowthami, Kuldeep Tripathi, Doddamalur Appaji Deepak, Kerur Vishwanath Raghavendra, Anuradha Agrawal","doi":"10.1007/s10722-024-02180-3","DOIUrl":null,"url":null,"abstract":"<p>The advancement of <i>Lathyrus</i> through genetic enhancement and cultivar development faces challenges due to limited knowledge about their reproductive biology. Thus, the present study systematically investigates the reproductive biology, phenology, receptivity of stigma, germination and viability of pollen in <i>Lathyrus sativus</i> L. and <i>L. cicera</i> L. Experiments were carried out using 20 accessions of <i>L. sativus</i> and three accessions of <i>L. cicera</i>. Anther dehiscence and stigma receptivity were determined through microscopic observation and hydrogen peroxide test, respectively. Pollen viability was assessed through in vitro pollen germination and fluorescein diacetate (FDA) test. Flowers were found to be bisexual and protogynous, with self-pollination favored by the cleistogamous nature of flowers. Five flower-development and three pod-development stages were classified in both species. The timing of all stages differed significantly among the accessions studied. Stigma receptivity was observed to occur 2–4 days before anther dehiscence and persisted until flower wilting. The study reveals that <i>L. sativus</i> and <i>L. cicera</i> are primarily self-pollinated, underscoring the critical timing of emasculation for successful manual crossing in breeding, ultimately enhancing pod and seed set, and facilitating plant breeders in optimizing hybridization programs.</p>","PeriodicalId":12467,"journal":{"name":"Genetic Resources and Crop Evolution","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic Resources and Crop Evolution","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10722-024-02180-3","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
The advancement of Lathyrus through genetic enhancement and cultivar development faces challenges due to limited knowledge about their reproductive biology. Thus, the present study systematically investigates the reproductive biology, phenology, receptivity of stigma, germination and viability of pollen in Lathyrus sativus L. and L. cicera L. Experiments were carried out using 20 accessions of L. sativus and three accessions of L. cicera. Anther dehiscence and stigma receptivity were determined through microscopic observation and hydrogen peroxide test, respectively. Pollen viability was assessed through in vitro pollen germination and fluorescein diacetate (FDA) test. Flowers were found to be bisexual and protogynous, with self-pollination favored by the cleistogamous nature of flowers. Five flower-development and three pod-development stages were classified in both species. The timing of all stages differed significantly among the accessions studied. Stigma receptivity was observed to occur 2–4 days before anther dehiscence and persisted until flower wilting. The study reveals that L. sativus and L. cicera are primarily self-pollinated, underscoring the critical timing of emasculation for successful manual crossing in breeding, ultimately enhancing pod and seed set, and facilitating plant breeders in optimizing hybridization programs.
期刊介绍:
Genetic Resources and Crop Evolution is devoted to all aspects of plant genetic resources research. It publishes original articles in the fields of taxonomical, morphological, physiological, biochemical, genetical, cytological or ethnobotanical research of genetic resources and includes contributions to gene-bank management in a broad sense, that means to collecting, maintenance, evaluation, storage and documentation.
Areas of particular interest include:
-crop evolution
-domestication
-crop-weed relationships
-related wild species
-history of cultivated plants including palaeoethnobotany.
Genetic Resources and Crop Evolution also publishes short communications, e.g. newly described crop taxa, nomenclatural notes, reports of collecting missions, evaluation results of gene-bank material etc. as well as book reviews of important publications in the field of genetic resources.
Every volume will contain some review articles on actual problems. The journal is the internationalized continuation of the German periodical Die Kulturpflanze, published formerly by the Institute of Plant Genetics and Crop Plant Research at Gatersleben, Germany.
All contributions are in the English language and are subject to peer reviewing.