Revolutionizing ELISA: A one-step blocking approach using lipid bilayer coatings

IF 7.2 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

Applied Materials Today Pub Date : 2024-08-26 DOI:10.1016/j.apmt.2024.102404

Kyongman An, Hyunhyuk Tae, Jihoon Shin, Jieun Bae, Sunguck Han, Gichan Choi, Tae-Hoon Kim, Nam-Joon Cho

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引用次数: 0

Abstract

Enzyme-linked immunosorbent assay (ELISA) is an essential technique for biomolecule detection in diagnostics and research, but traditional protocols are time-consuming and variable. Conventional blocking agents like bovine serum albumin (BSA) pose ethical issues and potential assay interference. This study presents a one-step lipid-blocking approach to simplify the ELISA procedure. Compared to conventional blockers, the Lipid Universal Coating Assembly (LUCA) antifouling blocker showed comparable sensitivity while significantly reducing processing time and steps. Furthermore, it showed improved co-blocking efficacy and signal intensity in conventional protocols when combined with minimal BSA concentrations. Stability tests under accelerated aging confirmed the robustness of the LUCA antifouling blocker. Additionally, it effectively facilitated antibody detection for COVID-19 antigens in direct ELISA and human IL-6 antigen in Sandwich ELISA, suggesting its versatile applicability. Taken together, our findings reveal that one-step lipid blocking not only streamlines the ELISA process but also maintains high sensitivity and specificity, providing an efficient, user-friendly, and environmentally friendly alternative to traditional ELISA blockers and a robust platform for rapid diagnostics.

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ELISA 革新：使用脂质双分子层涂层的一步阻断法

酶联免疫吸附测定法（ELISA）是诊断和研究中生物大分子检测的一项重要技术，但传统方法耗时长且易变。牛血清白蛋白（BSA）等传统阻断剂会带来道德问题和潜在的检测干扰。本研究提出了一种一步式脂质阻断方法，以简化 ELISA 程序。与传统的阻断剂相比，脂质通用涂层组件（LUCA）防污阻断剂的灵敏度相当，同时大大减少了处理时间和步骤。此外，当它与最低浓度的 BSA 结合使用时，在传统方案中显示出更好的协同阻断效果和信号强度。加速老化条件下的稳定性测试证实了 LUCA 防污阻断剂的稳定性。此外，它还有效地促进了直接酶联免疫吸附法（ELISA）中 COVID-19 抗原和三明治酶联免疫吸附法（ELISA）中人 IL-6 抗原的抗体检测，这表明它具有广泛的适用性。总之，我们的研究结果表明，一步脂质阻断不仅简化了 ELISA 过程，而且还保持了高灵敏度和特异性，为传统 ELISA 阻断剂提供了一种高效、用户友好和环保的替代品，并为快速诊断提供了一个强大的平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Applied Materials Today Materials Science-General Materials Science

CiteScore

14.90

自引率

3.60%

发文量

393

审稿时长

26 days

期刊介绍： Journal Name: Applied Materials Today Focus: Multi-disciplinary, rapid-publication journal Focused on cutting-edge applications of novel materials Overview: New materials discoveries have led to exciting fundamental breakthroughs. Materials research is now moving towards the translation of these scientific properties and principles.