Functional Studies of the Pollen-Specific Genes, StLAT52s in Potato

IF 2.3 3区 农林科学 Q1 AGRONOMY
Zhijun Han, Wenqian Yin, Binhuan Zhang, Yuzhu Han, Jingying Zhang
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Abstract

Pollen viability is a limiting factor in potato hybridization, although it varies considerably among potato varieties. Exploring the molecular basis of pollen viability will give a theoretical foundation for improving hybridization. In this research, we showed that the four StLAT52 genes, namely StLAT52a, StLAT52b, StLAT52c and StLAT52d were the key genes affecting potato pollen viability, and they were only expressed in flowers. It was also noted that these genes were localized in the nucleus. An assessment of protein–protein interactions showed that StLAT52a and StLAT52b proteins, StLAT52a and StLAT52c proteins, and StLAT52b and StLAT52c proteins interacted both in vitro and in vivo. When these genes were transferred into tobacco, the number of pollen grains significantly increased, and the pollen viability increased by almost 10%. In addition, the expression of the StLAT52 genes in tobacco resulted in significant changes in genes associated with the proteasome, ribosome biogenesis and plant hormone signal transduction pathways during the flowering stage. Taken together, this research provided an in-depth understanding of the structure and functions of the LAT52 genes in potatoes and their association with pollen viability. The results of this research can be used as a basis for further exploring the molecular mechanism underlying pollen viability.

Abstract Image

马铃薯花粉特异基因 StLAT52s 的功能研究
花粉活力是马铃薯杂交的一个限制因素,尽管不同马铃薯品种之间的差异很大。探索花粉活力的分子基础将为改进杂交提供理论依据。在这项研究中,我们发现四个 StLAT52 基因,即 StLAT52a、StLAT52b、StLAT52c 和 StLAT52d 是影响马铃薯花粉活力的关键基因,它们只在花中表达。研究还发现,这些基因定位于细胞核中。对蛋白质间相互作用的评估表明,StLAT52a 和 StLAT52b 蛋白、StLAT52a 和 StLAT52c 蛋白以及 StLAT52b 和 StLAT52c 蛋白在体外和体内都有相互作用。将这些基因转入烟草后,花粉粒数量显著增加,花粉活力提高了近 10%。此外,StLAT52 基因在烟草中的表达还导致与蛋白酶体、核糖体生物发生和植物激素信号转导途径相关的基因在开花期发生显著变化。总之,这项研究深入了解了马铃薯中 LAT52 基因的结构和功能及其与花粉活力的关系。该研究成果可作为进一步探索花粉活力分子机制的基础。
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来源期刊
Potato Research
Potato Research AGRONOMY-
CiteScore
5.50
自引率
6.90%
发文量
66
审稿时长
>12 weeks
期刊介绍: Potato Research, the journal of the European Association for Potato Research (EAPR), promotes the exchange of information on all aspects of this fast-evolving global industry. It offers the latest developments in innovative research to scientists active in potato research. The journal includes authoritative coverage of new scientific developments, publishing original research and review papers on such topics as: Molecular sciences; Breeding; Physiology; Pathology; Nematology; Virology; Agronomy; Engineering and Utilization.
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