Combined inoculation of arbuscular mycorrhiza fungi with Meso-rhizobium improves nutrient uptake, growth performance, and moisture stress tolerance of chickpea (Cicer arietinum L.)

Abstract

Biofertilizers can be better alternatives to chemical fertilizers to enhance plant nutrition and productivity as they improve the soil fertility and crop productivity in an eco-friendly and cost-effective manner. A pot experiment was conducted between December 2018 and March 2019 in southern Ethiopia to evaluate the combined inoculation of arbuscular mycorrhizal fungi (AMF) and Meso-rhizobium (MR) on biomass yield, nutrient uptake, and moisture stress tolerance of chickpea (Cicer arietinum L.) (variety: Habru). The experiment was executed as a factorial arrangement using a completely randomized design with three replications. The treatments were control (non-fertilized), sole AM fungi inoculation, AM fungi inoculation with phosphorus fertilizer (20 kg P ha⁻¹) and MR, and sole inorganic fertilizers (20 kg P;10 kg N ha⁻¹) at four different moisture levels (optimum throughout the growing season, stressed at vegetative, flowering, and seed filling stages). The results demonstrated that biomass yields were limited by moisture stress, especially at vegetative and flowering stages of chickpea. Sole and co-application of AMF with MR and inorganic P increased biomass yields on average by 19%, 39%, and 33% under water stress conditions, respectively, compared to the non-inoculated control. The application of AMF with MR and inorganic P also significantly increased nodulation, AMF colonization, and nutrient uptake, but these effects were dependent on soil moisture status. In conclusion, there are potential advantages to be gained from sole and combined AMF application with rhizobium to improve growth and productivity of chickpea through enhanced nutrient and water uptake, though the results of this pot experiment should be validated through field trials.