Dual-response fluorescent probe for lipid droplets and hypochlorous acid and imaging study in inflammation and atheromatous plaques

IF 4 2区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Molecular Structure Pub Date : 2024-09-12 DOI:10.1016/j.molstruc.2024.139994

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Abstract

Simultaneously detecting two biomarkers can significantly enhance disease diagnosis accuracy and deepen our understanding of disease pathogenesis. Developing a single fluorescent probe with two independent response units for this purpose is crucial. In line with this, a novel fluorescent probe (TPA-DT-DNH) had been proposed, featuring triphenylamine and C=N groups sensitive to lipid droplets (LDs) and hypochlorous acid (HClO), respectively. Spectral results highlighted that only the co-presence of LDs and HClO could “turn-on” the fluorescence of TPA-DT-DNH, when the concentration of one substance remained constant, the fluorescence intensity linearly increased with the concentration of the other substance. The imaging results demonstrated that TPA-DT-DNH can differentiate between normal cells and cells rich in LDs when HClO was present, and can detect HClO in HeLa cells. Furthermore, dynamic changes in the inflammation process induced by LPS can be visualized in both cells and zebrafish. Additionally, significant fluorescence enhancement was observed in atheromatous plaque.

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脂滴和次氯酸的双响应荧光探针以及炎症和动脉粥样斑块的成像研究

同时检测两种生物标记物可以大大提高疾病诊断的准确性，加深我们对疾病发病机制的了解。为此，开发具有两个独立响应单元的单一荧光探针至关重要。为此，我们提出了一种新型荧光探针（TPA-DT-DNH），其三苯胺基团和 C=N 基团分别对脂滴（LDs）和次氯酸（HClO）敏感。光谱结果表明，只有当脂滴和次氯酸同时存在时才能 "开启 "TPA-DT-DNH 的荧光，当其中一种物质的浓度保持不变时，荧光强度随另一种物质的浓度线性增加。成像结果表明，当 HClO 存在时，TPA-DT-DNH 能区分正常细胞和富含 LDs 的细胞，并能检测 HeLa 细胞中的 HClO。此外，细胞和斑马鱼都能观察到 LPS 诱导的炎症过程的动态变化。此外，在动脉粥样斑块中也观察到了明显的荧光增强。

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来源期刊

Journal of Molecular Structure 化学-物理化学

CiteScore

7.10

自引率

15.80%

发文量

2384

审稿时长

45 days

期刊介绍： The Journal of Molecular Structure is dedicated to the publication of full-length articles and review papers, providing important new structural information on all types of chemical species including: • Stable and unstable molecules in all types of environments (vapour, molecular beam, liquid, solution, liquid crystal, solid state, matrix-isolated, surface-absorbed etc.) • Chemical intermediates • Molecules in excited states • Biological molecules • Polymers. The methods used may include any combination of spectroscopic and non-spectroscopic techniques, for example: • Infrared spectroscopy (mid, far, near) • Raman spectroscopy and non-linear Raman methods (CARS, etc.) • Electronic absorption spectroscopy • Optical rotatory dispersion and circular dichroism • Fluorescence and phosphorescence techniques • Electron spectroscopies (PES, XPS), EXAFS, etc. • Microwave spectroscopy • Electron diffraction • NMR and ESR spectroscopies • Mössbauer spectroscopy • X-ray crystallography • Charge Density Analyses • Computational Studies (supplementing experimental methods) We encourage publications combining theoretical and experimental approaches. The structural insights gained by the studies should be correlated with the properties, activity and/ or reactivity of the molecule under investigation and the relevance of this molecule and its implications should be discussed.