Recoded gene circuits for multiplexed genetic code expansion

IF 33.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Nature biotechnology Pub Date : 2024-09-11 DOI:10.1038/s41587-024-02387-w

引用次数: 0

Abstract

Quadruplet codons allow multiplexing of non-canonical amino acids within single polypeptides in living cells. We show that including high-usage triplet codons after quadruplet codons can improve their decoding efficiency in genetic circuits, which allowed us to develop a system for the programmable biosynthesis of exotic macrocyclic peptides in cells.

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用于多路遗传密码扩展的重编码基因电路

在活细胞中，四重密码子可以复用单个多肽中的非规范氨基酸。我们的研究表明，在四重密码子之后加入高用量的三重密码子，可以提高基因电路的解码效率，从而开发出一种在细胞中可编程生物合成奇异大环肽的系统。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature biotechnology 工程技术-生物工程与应用微生物

CiteScore

63.00

自引率

1.70%

发文量

382

审稿时长

3 months

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