Insights into the functional mechanism of the non-specific lipid transfer protein nsLTP in Kalanchoe fedtschenkoi (Lavender scallops)

IF 1.4 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Protein expression and purification Pub Date : 2024-09-10 DOI:10.1016/j.pep.2024.106607

Dafeng Liu , Wenrui Dou , Hongying Song , Huashui Deng , Zhu Tian , Rong Chen , Zhen Liu , Ziwei Jiao , Oren Akhberdi

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引用次数: 0

Abstract

Plant non-specific lipid transfer protein (nsLTP) is able to bind and transport lipids and essential oils, as well as engage in various physiological processes, including defense against phytopathogens. Kalanchoe fedtschenkoi (Lavender Scallops) is an attractive and versatile succulent. To investigate the functional mechanism of Kalanchoe fedtschenkoi nsLTP (Ka-nsLTP), we expressed, purified and successfully obtained monomeric Ka-nsLTP. Mutational experiments revealed that the C6A variant retained the same activity as the wild-type (WT) Ka-nsLTP. Ka-nsLTP showed weak antiphytopathogenic bacterial activity, but inhibited fungal growth. Ka-nsLTP possessed a hydrophobic cavity effectively binding lauric acid. Our results offer novel molecular insights into the functional mechanism of nsLTP, which broadens our knowledge of the biological function of nsLTP in crops and provides a useful locus for genetic improvement of plants.

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对薰衣草扇贝中非特异性脂质转移蛋白 nsLTP 功能机制的认识

植物非特异性脂质转移蛋白（nsLTP）能够结合和运输脂质和精油，并参与各种生理过程，包括抵御植物病原体。薰衣草扇贝（Kalanchoe fedtschenkoi）是一种极具吸引力且用途广泛的多肉植物。为了研究 Kalanchoe fedtschenkoi nsLTP（Ka-nsLTP）的功能机制，我们表达、纯化并成功获得了单体 Ka-nsLTP。突变实验表明，C6A变体保留了与野生型（WT）Ka-nsLTP相同的活性。Ka-nsLTP 具有较弱的抗致病细菌活性，但能抑制真菌生长。Ka-nsLTP 具有一个疏水腔，能有效结合月桂酸。我们的研究结果为 nsLTP 的功能机制提供了新的分子见解，拓宽了我们对 nsLTP 在作物中生物功能的认识，为植物遗传改良提供了一个有用的基因位点。

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来源期刊

Protein expression and purification 生物-生化研究方法

CiteScore

3.70

自引率

6.20%

发文量

120

审稿时长

32 days

期刊介绍： Protein Expression and Purification is an international journal providing a forum for the dissemination of new information on protein expression, extraction, purification, characterization, and/or applications using conventional biochemical and/or modern molecular biological approaches and methods, which are of broad interest to the field. The journal does not typically publish repetitive examples of protein expression and purification involving standard, well-established, methods. However, exceptions might include studies on important and/or difficult to express and/or purify proteins and/or studies that include extensive protein characterization, which provide new, previously unpublished information.