Identification and functional analysis of recent IS transposition events in rhizobia.

IF 4.7 2区 生物学 Q1 GENETICS & HEREDITY
Ezequiel G Mogro, Walter O Draghi, Antonio Lagares, Mauricio J Lozano
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引用次数: 0

Abstract

Rhizobia are alpha- and beta- Proteobacteria that, through the establishment of symbiotic interactions with leguminous plants, are able to fix atmospheric nitrogen as ammonium. The successful establishment of a symbiotic interaction is highly dependent on the availability of nitrogen sources in the soil, and on the specific rhizobia strain. Insertion sequences (ISs) are simple transposable genetic elements that can move to different locations within the host genome and are known to play an important evolutionary role, contributing to genome plasticity by acting as recombination hot-spots, and disrupting coding and regulatory sequences. Disruption of coding sequences may have occurred either in a common ancestor of the species or more recently. By means of ISComapare, we identified Differentially Located ISs (DLISs) in nearly related rhizobial strains of the genera Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium. Our results revealed that recent IS transposition could have a role in adaptation by enabling the activation and inactivation of genes that could dynamically affect the competition and survival of rhizobia in the rhizosphere.

根瘤菌中近期 IS 转座事件的鉴定和功能分析。
根瘤菌是一种α-和β-变形菌,通过与豆科植物建立共生关系,能够将大气中的氮固定为铵态氮。共生作用的成功建立在很大程度上取决于土壤中氮源的可用性和特定的根瘤菌株。插入序列(ISs)是一种简单的可转座遗传因子,可移动到宿主基因组内的不同位置,在进化过程中发挥着重要作用,可作为重组热点,破坏编码和调控序列,从而促进基因组的可塑性。编码序列的破坏可能发生在物种的共同祖先或最近。通过 ISComapare,我们在根瘤菌属(Bradyrhizobium)、中根瘤菌属(Mesorhizobium)、根瘤菌属(Rhizobium)和中根瘤菌属(Sinorhizobium)的近亲根瘤菌菌株中鉴定了差异定位 IS(DLIS)。我们的研究结果表明,最近的IS转座可能在适应过程中发挥作用,使基因激活或失活,从而动态地影响根瘤菌在根瘤层中的竞争和生存。
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来源期刊
Mobile DNA
Mobile DNA GENETICS & HEREDITY-
CiteScore
8.20
自引率
6.10%
发文量
26
审稿时长
11 weeks
期刊介绍: Mobile DNA is an online, peer-reviewed, open access journal that publishes articles providing novel insights into DNA rearrangements in all organisms, ranging from transposition and other types of recombination mechanisms to patterns and processes of mobile element and host genome evolution. In addition, the journal will consider articles on the utility of mobile genetic elements in biotechnological methods and protocols.
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