Trabecular Meshwork Regeneration for Glaucoma Treatment Using Stem Cell-Derived Trophic Factors.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4087-6_4

Ajay Kumar, Enzhi Yang, Yiqin Du

引用次数: 0

Abstract

Glaucoma is one of the leading causes of irreversible blindness. Stem cell therapy has shown promise in the treatment of primary open-angle glaucoma in animal models. Stem cell-free therapy using stem cell-derived trophic factors might be in demand in patients with high-risk conditions or religious restrictions. In this chapter, we describe methods for trabecular meshwork stem cell (TMSC) cultivation, secretome harvesting, and protein isolation, as well as assays to ensure the health of TMSC post-secretome harvesting and for secretome periocular injection into mice for therapeutic purposes.

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利用干细胞衍生的营养因子再生小梁网治疗青光眼

青光眼是导致不可逆失明的主要原因之一。干细胞疗法已在动物模型中显示出治疗原发性开角型青光眼的前景。使用干细胞衍生营养因子的无干细胞疗法可能是高风险或宗教限制患者的需求。在本章中，我们介绍了小梁网状结构干细胞（TMSC）培养、分泌物采集和蛋白质分离的方法，以及确保分泌物采集后TMSC健康的检测方法和将分泌物注射到小鼠眼周以达到治疗目的的检测方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.