The mechanical properties measurement could be affected by forceps: a technical note on sampling human annulus fibrosus.

IF 2.6 3区医学 Q2 CLINICAL NEUROLOGY

European Spine Journal Pub Date : 2024-11-01 Epub Date: 2024-09-06 DOI:10.1007/s00586-024-08438-9

Tianchi Zhou, Bowei Xiao, Juying Huang, Tianhua Rong, Bingxuan Wu, Baoge Liu

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引用次数: 0

Abstract

Purpose: To compare the mechanical properties of human annulus fibrosus obtained by forceps versus bistoury and observe whether the measurement could be affected by forceps sampling method.

Methods: In this study, the mechanical properties of the the extracellular matrix (ECM) of human annulus fibrosus, including elastic modulus and stiffness, were investigated using atomic force microscope (AFM). Tissue was obtained from patients during operation using a bistoury or nucleus pulposus forceps. Tissues obtained with the nucleus pulposus forceps were considered as the forceps group and those obtained with a bistoury were considered as the bistoury group.

Results: There was no significant difference observed between the forceps and bistoury group according to histological staining. The elastic modulus of the forceps group was 0.41 ± 0.08 MPa, and that of bistoury group was 0.53 ± 0.13 MPa, and the difference between the two groups was statistically significant (p < 0.05). The stiffness of the forceps group was 0.024 ± 0.003 N/m, and that of the bistoury group was 0.037 ± 0.003 N/m, and the difference between the two groups was statistically significant (p < 0.05).

Conclusion: The results indicate that the forceps sampling method has a substantial negative effect on the micromechanical properties of the ECM of the annulus fibrosus. Bistoury sampling method is recommended as the experimental subject for exploring the micromechanics mechanisms of cervical degenerative disease.

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机械性能测量可能受镊子影响：人体纤维环取样技术说明。

目的：比较用镊子取样和用双套管取样获得的人体纤维环的力学性能，并观察镊子取样方法是否会影响测量结果：本研究使用原子力显微镜（AFM）研究了人纤维环细胞外基质（ECM）的力学特性，包括弹性模量和刚度。组织是在手术过程中使用活瓣钳或髓核钳从患者身上获取的。使用髓核钳获取的组织被视为髓核钳组，使用双镊获取的组织被视为双镊组：结果：根据组织学染色，镊子组和双盘组之间没有明显差异。镊子取样组的弹性模量为 0.41 ± 0.08 MPa，镊子取样组的弹性模量为 0.53 ± 0.13 MPa，两组间差异有统计学意义（P 结论：镊子取样组和镊子取样组的弹性模量差异无统计学意义（P>0.05）：结果表明，镊子取样法对纤维环 ECM 的微机械性能有很大的负面影响。建议将 Bistoury 取样法作为探索颈椎退行性疾病微观力学机制的实验对象。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Spine Journal 医学-临床神经学

CiteScore

4.80

自引率

10.70%

发文量

373

审稿时长

2-4 weeks

期刊介绍： "European Spine Journal" is a publication founded in response to the increasing trend toward specialization in spinal surgery and spinal pathology in general. The Journal is devoted to all spine related disciplines, including functional and surgical anatomy of the spine, biomechanics and pathophysiology, diagnostic procedures, and neurology, surgery and outcomes. The aim of "European Spine Journal" is to support the further development of highly innovative spine treatments including but not restricted to surgery and to provide an integrated and balanced view of diagnostic, research and treatment procedures as well as outcomes that will enhance effective collaboration among specialists worldwide. The “European Spine Journal” also participates in education by means of videos, interactive meetings and the endorsement of educative efforts. Official publication of EUROSPINE, The Spine Society of Europe