Metabolic Profiling of Rat Kidney Tissue Following Administration of D-Allulose.

IF 1.2 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Journal of applied glycoscience Pub Date : 2024-08-20 eCollection Date: 2024-01-01 DOI:10.5458/jag.jag.JAG-2023_0019
Akane Kanasaki, Misato Niibo, Tetsuo Iida
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引用次数: 0

Abstract

D-Allulose (D-psicose) is a rare sugar and a C-3 epimer of D-fructose. D-Allulose has been reported to have several health benefits via its alteration of both glucose and lipid metabolism. It was previously reported that D-allulose alters the hepatic metabolomic profile. Although the kidneys are crucial organs in metabolic regulation, the effects of D-allulose on renal metabolism have not yet been established. Therefore, this study was designed to capture the overall metabolic response in the kidneys to D-allulose. This was done by providing an AIN-93G diet to Wistar rats, with or without 3 % D-allulose, for four weeks. Renal tissue and blood samples were collected after a 3-hour fasting for evaluation of the renal metabolic profile and their related plasma parameters. D-Allulose increased renal weight without changes in the plasma indices associated with reduced renal function. Metabolic profiling identified a total of 264 peaks. As the contribution rate was too low in the principal component analysis results of the metabolic profiling results, we evaluated the metabolites that were significantly different between two groups and identified 23 up-regulated and 26 down-regulated metabolites in the D-allulose group. D-Allulose also had significant influence on several metabolites involved in glucose metabolism, amino acid metabolism, and purine metabolism. Moreover, the levels of trimethylamine N-oxide and symmetric dimethylarginine, which are associated with several diseases such as chronic kidney disease and cardiovascular disease decreased following D-allulose diets. This study showed that D-allulose affects the renal metabolic profile, and our findings will help elucidate the function of D-allulose.

服用 D-阿洛糖后大鼠肾组织的代谢谱分析
D-阿洛糖(D-糙米糖)是一种稀有糖类,也是 D-果糖的 C-3 二聚体。据报道,D-阿洛糖通过改变葡萄糖和脂质代谢对健康有多种益处。以前曾有报道称,D-阿洛糖能改变肝脏代谢组谱。虽然肾脏是代谢调节的关键器官,但 D-阿洛糖对肾脏代谢的影响尚未确定。因此,本研究旨在捕捉肾脏对 D-阿洛糖的整体代谢反应。具体方法是为 Wistar 大鼠提供含有或不含有 3% D-阿洛糖的 AIN-93G 食物,为期四周。空腹 3 小时后收集肾组织和血液样本,以评估肾脏代谢概况及其相关血浆参数。D-阿洛糖增加了肾脏重量,但与肾功能减退相关的血浆指标没有发生变化。代谢图谱共发现了 264 个峰值。由于代谢轮廓分析结果中主成分分析结果的贡献率太低,我们对两组之间存在显著差异的代谢物进行了评估,发现在 D-阿洛糖组中有 23 个上调代谢物和 26 个下调代谢物。D-阿洛糖还对涉及葡萄糖代谢、氨基酸代谢和嘌呤代谢的多个代谢物产生了显著影响。此外,与慢性肾病和心血管疾病等多种疾病相关的三甲胺 N-氧化物和对称二甲基精氨酸的水平在 D-阿洛糖膳食后也有所下降。这项研究表明,D-阿洛糖会影响肾脏代谢状况,我们的研究结果将有助于阐明 D-阿洛糖的功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of applied glycoscience
Journal of applied glycoscience BIOCHEMISTRY & MOLECULAR BIOLOGY-
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9.10%
发文量
13
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