Effect of ohmic heating on the extraction of biocompounds from aqueous and ethanolic suspensions of Pavlova gyrans

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing Pub Date : 2024-08-23 DOI:10.1016/j.fbp.2024.08.008

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引用次数: 0

Abstract

Electric field technology has been highlighted as a promising strategy in the permeabilization of cell membranes and the corresponding bioactive release. In this work, aqueous and ethanolic suspensions of Pavlova gyrans were subjected to moderate electric fields (MEF) to promote rapid heating due to Ohmic Heating (OH) effect. Two approaches were tested: i) OH assisted extraction in a temperature range between 25 ºC and 85 ºC, and ii) OH pretreatment at 25 ºC and 55 ºC followed by solvent extraction. OH treatment at 55 ºC (performed in less than 10 s) in aqueous suspensions promoted a marked increase in the release of organic matter (2.1-fold), chlorophyll a (41.1-fold) and total carotenoids (56.6-fold) when compared to the freeze-thaw cycles (FTC ≈ 3 hours). OH-pretreated biomass subjected to a two-step passive extraction improved the release of proteins and chlorophylls over incubation (p < 0.05) when compared to untreated biomass. This procedure increased 2.2-, 3.9- and 31.9-fold the content of organic matter, protein and chlorophyll a, respectively, in comparison to the FTC procedure. This work presented the OH effect as a promising strategy for enhanced disruption and release of intracellular microalgae compounds.

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欧姆加热对从 Pavlova gyrans 的水悬浮液和乙醇悬浮液中提取生物化合物的影响

电场技术已被视为细胞膜渗透和相应生物活性释放的一种有前途的策略。在这项工作中，将 Pavlova gyrans 的水悬浮液和乙醇悬浮液置于中等电场（MEF）中，以促进欧姆加热（OH）效应引起的快速加热。试验采用了两种方法：i) 在 25 ºC 至 85 ºC 的温度范围内进行 OH 辅助萃取；ii) 在 25 ºC 和 55 ºC 温度范围内进行 OH 预处理，然后进行溶剂萃取。与冻融循环（FTC ≈ 3 小时）相比，在 55 ºC 下对水悬浮液进行 OH 处理（处理时间少于 10 秒）可显著增加有机物（2.1 倍）、叶绿素 a（41.1 倍）和类胡萝卜素总量（56.6 倍）的释放。与未经处理的生物质相比，经过两步被动萃取的 OH 预处理生物质在培养过程中提高了蛋白质和叶绿素的释放量（p < 0.05）。与 FTC 程序相比，该程序使有机物、蛋白质和叶绿素 a 的含量分别增加了 2.2 倍、3.9 倍和 31.9 倍。这项工作表明，OH 效应是增强细胞内微藻化合物的破坏和释放的一种有前途的策略。

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来源期刊

Food and Bioproducts Processing 工程技术-工程：化工

CiteScore

9.70

自引率

4.30%

发文量

115

审稿时长

24 days

期刊介绍： Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.