Comparison of Zybio Kit and saponin in-house method in rapid identification of bacteria from positive blood cultures by EXS2600 matrix-assisted laser desorption ionization time-of-flight mass spectrometry system

IF 1.9 3区 化学 Q3 BIOCHEMICAL RESEARCH METHODS
Mislav Peras, Ivana Mareković, Tomislav Kuliš, Manda Markanović, Ana Budimir
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引用次数: 0

Abstract

We evaluated the performance of Zybio EXS2600 matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) (Zybio Inc., Chongqing, China) for the identification of bacteria from positive blood culture (BC) bottles using Blood Culture Positive Sample Pretreatment Kit (Zybio Inc., Chongqing, China) in comparison to an in-house saponin method. Following a positive signal by the BACTEC™ FX system, confirmation of identification was achieved using subcultured growing biomass used for MALDI-TOF MS analysis. A total of 94 positive BC bottles with 97 bacterial isolates were analyzed. The overall identification rates at the genus and species levels for the saponin method were 89.7% (87/97) and 74.2% (72/97), respectively. With the Zybio Kit, 88.7% (86/97) and 80.4% (78/97) of microorganisms were correctly identified to the genus and species levels, respectively. The saponin method identified 65.3% (32/49) of Gram-positive bacteria at the species level, whereas the Zybio Kit achieved a higher species-level identification rate of 79.6% (39/49) (p = 0.1153). The saponin method with additional on-plate formic acid extraction showed a significantly higher overall identification rate in comparison to the saponin method without that step for both genus (87.6% [85/97] vs. 70.1% [68/97], p = 0.0029) and species level (70.1% [68/97] vs. 46.4% [45/97], p = 0.0008). Identification rates of Gram-negative bacteria showed a higher identification rate, however, not statistically significant with additional Zybio Kit protocol step on both genus (85.4% [41/48] vs. 81.3% [39/48], p = 0.5858) and species level (77.1% [37/48] vs. 75% [36/48], p = 0.8120). Zybio Kit could offer an advantage in species-level identification, particularly for Gram-positive bacteria. The inclusion of on-plate formic acid extraction in the saponin method notably enhanced identification at both genus and species levels for Gram-positive bacteria. The extended protocol provided by the Zybio Kit could potentially offer an advantage in the identification of Gram-negative bacteria at both genus and species levels. Enhancements to the Zybio EXS2600 MALDI-TOF instrument software database are necessary.

使用 EXS2600 矩阵辅助激光解吸电离飞行时间质谱系统快速鉴定阳性血液培养物中细菌的 Zybio Kit 与皂素内部方法的比较。
我们评估了Zybio EXS2600基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)(Zybio Inc.,重庆,中国)与内部皂素方法的性能比较,后者可用于使用血培养阳性样品预处理试剂盒(Zybio Inc.,重庆,中国)鉴定血培养(BC)阳性瓶中的细菌。在 BACTEC™ FX 系统检测到阳性信号后,使用用于 MALDI-TOF MS 分析的亚培养生长生物质进行鉴定确认。共分析了 94 个阳性 BC 瓶和 97 个细菌分离物。皂素法的属种鉴定率分别为 89.7%(87/97)和 74.2%(72/97)。使用 Zybio Kit,分别有 88.7%(86/97)和 80.4%(78/97)的微生物被正确鉴定为属和种。皂素法可鉴定出 65.3%(32/49)的革兰氏阳性细菌的种,而 Zybio 套件的种鉴定率更高,达到 79.6%(39/49)(p = 0.1153)。在属级(87.6% [85/97] vs. 70.1% [68/97],p = 0.0029)和种级(70.1% [68/97] vs. 46.4% [45/97],p = 0.0008)的总体鉴定率上,皂素法与额外的板上甲酸提取法相比明显更高。革兰氏阴性菌的鉴定率较高,但在属级(85.4% [41/48] vs. 81.3% [39/48],p = 0.5858)和种级(77.1% [37/48] vs. 75% [36/48],p = 0.8120)增加 Zybio Kit 方案步骤后,鉴定率并无统计学意义。Zybio Kit 在物种鉴定方面具有优势,尤其是对革兰氏阳性菌。在皂苷方法中加入板上甲酸提取,可显著提高革兰氏阳性菌属和种的鉴定水平。Zybio 试剂盒提供的扩展方案有可能在革兰氏阴性菌的属种鉴定方面提供优势。有必要对 Zybio EXS2600 MALDI-TOF 仪器软件数据库进行改进。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Mass Spectrometry
Journal of Mass Spectrometry 化学-光谱学
CiteScore
5.10
自引率
0.00%
发文量
84
审稿时长
1.5 months
期刊介绍: The Journal of Mass Spectrometry publishes papers on a broad range of topics of interest to scientists working in both fundamental and applied areas involving the study of gaseous ions. The aim of JMS is to serve the scientific community with information provided and arranged to help senior investigators to better stay abreast of new discoveries and studies in their own field, to make them aware of events and developments in associated fields, and to provide students and newcomers the basic tools with which to learn fundamental and applied aspects of mass spectrometry.
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