Transcriptomic analysis of meiotic genes during the mitosis-to-meiosis transition in Drosophila females.

IF 3.3 3区生物学 Q2 GENETICS & HEREDITY

Genetics Pub Date : 2024-09-03 DOI:10.1093/genetics/iyae130

Ana Maria Vallés, Thomas Rubin, Nicolas Macaisne, Laurine Dal Toe, Anahi Molla-Herman, Christophe Antoniewski, Jean-René Huynh

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Abstract

Germline cells produce gametes, which are specialized cells essential for sexual reproduction. Germline cells first amplify through several rounds of mitosis before switching to the meiotic program, which requires specific sets of proteins for DNA recombination, chromosome pairing, and segregation. Surprisingly, we previously found that some proteins of the synaptonemal complex, a prophase I meiotic structure, are already expressed and required in the mitotic region of Drosophila females. Here, to assess if additional meiotic genes were expressed earlier than expected, we isolated mitotic and meiotic cell populations to compare their RNA content. Our transcriptomic analysis reveals that all known meiosis I genes are already expressed in the mitotic region; however, only some of them are translated. As a case study, we focused on mei-W68, the Drosophila homolog of Spo11, to assess its expression at both the mRNA and protein levels and used different mutant alleles to assay for a premeiotic function. We could not detect any functional role for Mei-W68 during homologous chromosome pairing in dividing germ cells. Our study paves the way for further functional analysis of meiotic genes expressed in the mitotic region.

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雌果蝇有丝分裂至减数分裂过渡期减数分裂基因的转录组分析

生殖细胞产生配子，配子是有性生殖所必需的特化细胞。生殖细胞首先经过几轮有丝分裂扩增，然后转入减数分裂程序，该程序需要特定的蛋白质来进行DNA重组、染色体配对和分离。令人惊讶的是，我们之前发现，减数分裂前期结构--突触复合体的一些蛋白质已经在雌果蝇的有丝分裂区表达和需要。在这里，为了评估是否有更多的减数分裂基因比预期的更早表达，我们分离了有丝分裂细胞群和减数分裂细胞群，以比较它们的 RNA 含量。我们的转录组分析表明，所有已知的减数分裂 I 基因都已在有丝分裂区表达，但只有部分基因被翻译。作为一个案例研究，我们重点研究了果蝇 Spo11 的同源物 mei-W68，以评估其在 mRNA 和蛋白质水平上的表达情况，并使用不同的突变等位基因来检测其减数分裂前的功能。我们无法检测到Mei-W68在分裂生殖细胞中同源染色体配对过程中的任何功能作用。我们的研究为进一步分析在有丝分裂区表达的减数分裂基因铺平了道路。

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来源期刊

Genetics GENETICS & HEREDITY-

CiteScore

6.90

自引率

6.10%

发文量

177

审稿时长

1.5 months

期刊介绍： GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.