Abundant mRNA m¹A modification in dinoflagellates: a new layer of gene regulation.

IF 6.5 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

EMBO Reports Pub Date : 2024-11-01 Epub Date: 2024-09-02 DOI:10.1038/s44319-024-00234-2

Chongping Li, Ying Li, Jia Guo, Yuci Wang, Xiaoyan Shi, Yangyi Zhang, Nan Liang, Honghui Ma, Jie Yuan, Jiawei Xu, Hao Chen

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引用次数: 0

Abstract

Dinoflagellates, a class of unicellular eukaryotic phytoplankton, exhibit minimal transcriptional regulation, representing a unique model for exploring gene expression. The biosynthesis, distribution, regulation, and function of mRNA N1-methyladenosine (m¹A) remain controversial due to its limited presence in typical eukaryotic mRNA. This study provides a comprehensive map of m¹A in dinoflagellate mRNA and shows that m¹A, rather than N6-methyladenosine (m⁶A), is the most prevalent internal mRNA modification in various dinoflagellate species, with an asymmetric distribution along mature transcripts. In Amphidinium carterae, we identify 6549 m¹A sites characterized by a non-tRNA T-loop-like sequence motif within the transcripts of 3196 genes, many of which are involved in regulating carbon and nitrogen metabolism. Enriched within 3'UTRs, dinoflagellate mRNA m¹A levels negatively correlate with translation efficiency. Nitrogen depletion further decreases mRNA m¹A levels. Our data suggest that distinctive patterns of m¹A modification might influence the expression of metabolism-related genes through translational control.

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甲藻中丰富的 mRNA m1A 修饰：基因调控的新层次。

甲藻是一类单细胞真核浮游植物，表现出极少的转录调控，是探索基因表达的独特模型。由于 mRNA N1-甲基腺苷（m1A）在典型真核生物 mRNA 中的存在有限，因此其生物合成、分布、调控和功能仍存在争议。这项研究提供了甲藻 mRNA 中 m1A 的全面分布图，并表明 m1A 而不是 N6-甲基腺苷（m6A）是各种甲藻中最普遍的 mRNA 内部修饰，而且沿着成熟转录本呈不对称分布。在Amphidinium carterae中，我们在3196个基因的转录本中发现了6549个m1A位点，其特征是非tRNA T-环状序列图案，其中许多基因参与调控碳和氮的代谢。甲藻 mRNA m1A 富集在 3'UTR 中，其水平与翻译效率呈负相关。氮耗竭会进一步降低 mRNA m1A 水平。我们的数据表明，m1A修饰的独特模式可能会通过翻译控制影响代谢相关基因的表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

EMBO Reports 生物-生化与分子生物学

CiteScore

11.20

自引率

1.30%

发文量

267

审稿时长

1 months

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