splicekit: an integrative toolkit for splicing analysis from short-read RNA-seq.

IF 2.4 Q2 MATHEMATICAL & COMPUTATIONAL BIOLOGY
Bioinformatics advances Pub Date : 2024-08-17 eCollection Date: 2024-01-01 DOI:10.1093/bioadv/vbae121
Gregor Rot, Arne Wehling, Roland Schmucki, Nikolaos Berntenis, Jitao David Zhang, Martin Ebeling
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引用次数: 0

Abstract

Motivation: Analysis of alternative splicing using short-read RNA-seq data is a complex process that involves several steps: alignment of reads to the reference genome, identification of alternatively spliced features, motif discovery, analysis of RNA-protein binding near donor and acceptor splice sites, and exploratory data visualization. To the best of our knowledge, there is currently no integrative open-source software dedicated to this task.

Results: Here, we introduce splicekit, a Python package that provides and integrates a set of existing and novel splicing analysis tools for conducting splicing analysis.

Availability and implementation: The software splicekit is open-source and available at Github (https://github.com/bedapub/splicekit) and via the Python Package Index.

splicekit:从短线程 RNA-seq 进行剪接分析的综合工具包。
动机:利用短线程 RNA-seq 数据分析替代剪接是一个复杂的过程,涉及多个步骤:将读数与参考基因组比对、识别替代剪接特征、发现主题、分析供体和受体剪接位点附近的 RNA 蛋白结合以及探索性数据可视化。据我们所知,目前还没有专门用于这项任务的集成式开源软件:在此,我们介绍了 splicekit,它是一个 Python 软件包,提供并集成了一套现有的和新颖的剪接分析工具,用于进行剪接分析:软件 splicekit 是开源的,可通过 Github (https://github.com/bedapub/splicekit) 和 Python 软件包索引获取。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
1.60
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